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Invitrogen
alpha-2c Adrenergic Receptor Monoclonal Antibody (N330A/80)
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FIGURE: 1 / 2
alpha-2c Adrenergic Receptor Antibody (MA5-27724) in ICC/IF
Immunofluorescent analysis of alpha-2C Adrenergic Receptor in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with alpha-2C Adrenergic Receptor monoclonal antibody (Product # MA5-27724) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min ... View More
Product Details
MA5-27724
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N330A/80
Immunogen
Synthetic peptide amino acids 442- 462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27724 was sufficient for detection of Alpha2C Adrenergic Receptor in 20 µg of COS cells transiently transfected with HA-tagged Alpha- lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |Detects 50kDa or larger (possibly due to dimerization). Does not cross-react with other adrenergic receptors.
This antibody was formerly sold as clone S330A-80.
Target Information
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adra 2c; adrenergic, alpha-2C-, receptor; Alpha 2CAR; Alpha-2 adrenergic receptor subtype C4; Alpha-2C adrenergic receptor; Alpha-2C adrenoceptor; Alpha-2C adrenoreceptor; alpha-2C-adrenergic receptor; Alpha-2C-AR; alpha-2CAR; alpha2 AR C4; alpha2 C4; alpha2-AR-C4; subtype alpha2-C4
Gene Aliases: [a]2C; Adra-2c; ADRA2C; ADRA2L2; ADRA2RL2; ADRARL2; alpha2-C4; alpha2C; ALPHA2CAR
UniProt ID: (Human) P18825, (Mouse) Q01337
Entrez Gene ID: (Human) 152, (Mouse) 11553
G-protein coupled receptor signaling pathway
cell-cell signaling
female pregnancy
negative regulation of norepinephrine secretion
platelet activation
activation of protein kinase B activity
negative regulation of epinephrine secretion
epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway
positive regulation of neuron differentiation
positive regulation of vasoconstriction
regulation of insulin secretion
regulation of sensory perception of pain
negative regulation of uterine smooth muscle contraction
adenylate cyclase-activating adrenergic receptor signaling pathway
activation of MAPK activity by adrenergic receptor signaling pathway
regulation of smooth muscle contraction
signal transduction
negative regulation of adenylate cyclase activity
regulation of vasoconstriction
negative regulation of insulin secretion involved in cellular response to glucose stimulus
adrenergic receptor signaling pathway
adenylate cyclase-inhibiting adrenergic receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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