Invitrogen

Phospho-MEK1/MEK2 (Ser221) Recombinant Rabbit Monoclonal Antibody (MEK12S221-D3), APC

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Phospho-MEK1/MEK2 (Ser221) Antibody in Flow Cytometry (Flow)

Phospho-MEK1/MEK2 (Ser221) Antibody in Flow Cytometry (Flow)

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Phospho-MEK1/MEK2 (Ser221) Antibody (MA5-37079) in Flow

Flow cytometry of Phospho-MEK1/2 (Ser221) using HeLa cells. Samples were incubated in monoclonal Phospho-MEK1/2 (Ser221) antibody (Product # MA5-37079). Images correspond with the following conditions: treated with imatinib and unstained as negative control (blue), treated with imatinib (red), with pervanadate (green).

Phospho-MEK1/MEK2 (Ser221) Antibody in Flow Cytometry (Flow)

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Phospho-MEK1/MEK2 (Ser221) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-MEK1/2 (Ser221) using HeLa cells. Samples were incubated in monoclonal Phospho-MEK1/2 (Ser221) antibody (Product # MA5-37079). Images correspond with the following conditions: treated with imatinib and unstained as negative control (blue), treated with imatinib (red), with pervanadate (green).

产品信息

MA5-37079

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL/1x10^6 cells

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

MEK12S221-D3

抗原

A synthetic phospho-peptide corresponding to residues surrounding Ser221 of human phospho MEK1/2.

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偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

纯化类型

Protein A/G

保存液

PBS with 0.20% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2897014

靶标信息

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). In brain, this pathway also plays a key role in synaptic (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 2; EC 2.7.12.2; ERK activator kinase 1; ERK activator kinase 2; kinase MEK1; MAP kinase kinase 1; MAP kinase kinase 2; MAP kinase/Erk kinase; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MEK 1; MEK 2; MEK-1; MEK-2; mitogen activated protein kinase kinase 1; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MP2K1; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen activated, kinase 2, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

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基因别名: AA589381; CFC3; CFC4; MAP2K1; MAP2K2; MAPKK1; MAPKK2; MEK1; MEK2; MEKK1; MK2; MKK1; MKK2; PRKMK1; PRKMK2

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UniProt ID: (Human) Q02750, (Human) P36507, (Mouse) P31938, (Mouse) Q63932

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Entrez Gene ID: (Human) 5604, (Human) 5605, (Mouse) 26395, (Mouse) 26396

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protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity MAP kinase kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity receptor signaling protein tyrosine phosphatase activity protein binding ATP binding protein C-terminus binding protein kinase binding protein serine/threonine kinase activator activity protein N-terminus binding PDZ domain binding protein complex scaffold metal ion binding scaffold protein binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Ras GTPase binding mitogen-activated protein kinase kinase kinase binding non-receptor serine/threonine protein kinase

参与通路

MAPK cascade activation of MAPK activity movement of cell or subcellular component chemotaxis cell cycle arrest signal transduction heart development negative regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression peptidyl-tyrosine phosphorylation cerebellar cortex formation neuron differentiation keratinocyte differentiation thyroid gland development regulation of stress-activated MAPK cascade proteolysis in other organism positive regulation of transcription, DNA-templated thymus development regulation of axon regeneration cell motility positive regulation of axonogenesis Bergmann glial cell differentiation face development trachea formation epithelial cell proliferation involved in lung morphogenesis placenta blood vessel development labyrinthine layer development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of protein serine/threonine kinase activity regulation of Golgi inheritance cellular senescence mitophagy in response to mitochondrial depolarization positive regulation of production of miRNAs involved in gene silencing by miRNA regulation of early endosome to late endosome transport peptidyl-serine autophosphorylation positive regulation of cell motility regulation of protein phosphorylation regulation of vascular smooth muscle contraction protein phosphorylation response to oxidative stress mitotic nuclear division cell proliferation phosphorylation positive regulation of cell migration melanosome transport positive regulation of transcription elongation from RNA polymerase II promoter negative regulation of homotypic cell-cell adhesion peptidyl-tyrosine dephosphorylation positive regulation of GTPase activity positive regulation of cell differentiation positive regulation of Ras protein signal transduction vesicle transport along microtubule Golgi inheritance response to axon injury neuron projection morphogenesis protein heterooligomerization response to glucocorticoid lung morphogenesis