Invitrogen

Phospho-NFkB p65 (Ser529) Recombinant Rabbit Monoclonal Antibody (NFkBp65S529-H3)

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抗体检测数据 (3)

Phospho-NFkB p65 (Ser529) Antibody in Flow Cytometry (Flow)

Phospho-NFkB p65 (Ser529) Antibody in Flow Cytometry (Flow)

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Phospho-NFkB p65 (Ser529) Antibody (MA5-37164) in Flow

Flow cytometry of Phospho-NFkB p65 (Ser529) using HeLa cells. Samples were incubated in monoclonal Phospho-NFkB p65 (Ser529) antibody (Product # MA5-37164) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (light blue), untreated (red), treated with TNFa/calyculin A (green), untreated and blocked with phospho-peptide (black), treated and blocked with phospho pe... View More

Phospho-NFkB p65 (Ser529) Antibody in Flow Cytometry (Flow)

Phospho-NFkB p65 (Ser529) Antibody in Flow Cytometry (Flow)

Phospho-NFkB p65 (Ser529) Antibody in Flow Cytometry (Flow)

Product Image Gallery

Phospho-NFkB p65 (Ser529) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-NFkB p65 (Ser529) using HeLa cells. Samples were incubated in monoclonal Phospho-NFkB p65 (Ser529) antibody (Product # MA5-37164) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (light blue), untreated (red), treated with TNFa/calyculin A (green), untreated and blocked with phospho-peptide (black), treated and blocked with phospho peptide (gold), untreated and blocked with non-phospho peptide (dark blue), treated and blocked with non-phospho peptide (purple).

Phospho-NFkB p65 (Ser529) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-NFkB p65 (Ser529) using HeLa cells. Samples were incubated in monoclonal Phospho-NFkB p65 (Ser529) antibody (Product # MA5-37164) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (blue), untreated (red), treated with TNFa/calyculin A (green).

Phospho-NFkB p65 (Ser529) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-NFkB p65 (Ser529) using C2C12 cells. Samples were incubated in monoclonal Phospho-NFkB p65 (Ser529) antibody (Product # MA5-37164) with a dilution of 1 µg/mL. Images correspond with the following conditions: negative control (blue), treated with imatinib (red), with pervanadate (green).

产品信息

MA5-37164

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1-1,000 ng/mL

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

NFkBp65S529-H3

抗原

A synthetic phospho-peptide corresponding to residues surrounding Ser529 of human phospho-NFKB p65.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 0.10% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2897098

靶标信息

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kB p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; REL A; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A

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基因别名: NFKB3; p65; RELA

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UniProt ID: (Human) Q04206, (Mouse) Q04207

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Entrez Gene ID: (Human) 5970, (Mouse) 19697

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RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding protein complex binding activating transcription factor binding phosphate ion binding identical protein binding protein homodimerization activity actinin binding histone deacetylase binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding NF-kappaB binding repressing transcription factor binding ankyrin repeat binding enzyme binding sequence-specific DNA binding Rel homology transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter liver development hair follicle development stimulatory C-type lectin receptor signaling pathway acetaldehyde metabolic process transcription from RNA polymerase II promoter inflammatory response cellular defense response aging positive regulation of cell proliferation organ morphogenesis response to organic substance response to UV-B positive regulation of Schwann cell differentiation cytokine-mediated signaling pathway membrane protein intracellular domain proteolysis positive regulation of chondrocyte differentiation positive regulation of type I interferon production response to muramyl dipeptide response to progesterone response to insulin response to cobalamin cellular response to hepatocyte growth factor stimulus response to muscle stretch Fc-epsilon receptor signaling pathway negative regulation of protein catabolic process response to drug negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid response to morphine positive regulation of interleukin-12 biosynthetic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin receptor signaling pathway regulation of inflammatory response T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity response to cAMP defense response to virus cellular response to hydrogen peroxide nucleotide-binding oligomerization domain containing 2 signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to nicotine cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor cellular response to peptide hormone stimulus negative regulation of NIK/NF-kappaB signaling positive regulation of miRNA metabolic process negative regulation of extrinsic apoptotic signaling pathway transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response I-kappaB kinase/NF-kappaB signaling response to bacterium response to cytokine NIK/NF-kappaB signaling innate immune response