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PTPRF Antibody (MA5-45637) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with LAR/PTPRF monoclonal antibody (Product # MA5-45637) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stai... View More
产品信息
MA5-45637
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
N165/38
抗原
Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR.
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932091
产品详细信息
97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.
1 µg/mL of MA5-45637 was sufficient for detection of LAR/PTPRF in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 85kDa (full length protein is 210 kDa - smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.
This antibody was formerly sold as clone S165-38.
靶标信息
Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase).The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ43335; FLJ45062; FLJ45567; LAR; LAR protein; LCA homolog; LCA-homolog; leukocyte antigen-related (LAR) PTP receptor; leukocyte antigen-related tyrosine phosphatase; Leukocyte common antigen related; protein tyrosine phosphatase receptor-type F; protein tyrosine phosphatase, receptor type, F polypeptide; PTPRF protein; receptor-linked protein-tyrosine phosphatase LAR; Receptor-type tyrosine-protein phosphatase F
基因别名: AA591035; BNAH2; LAR; LARFN5C; LARS; PTPRF; RPTP-LAR
UniProt ID: (Human) P10586, (Rat) Q64604, (Mouse) A2A8L5
Entrez Gene ID: (Human) 5792, (Rat) 360406, (Mouse) 19268
protein tyrosine phosphatase activity
transmembrane receptor protein tyrosine phosphatase activity
heparin binding
protein complex binding
chondroitin sulfate proteoglycan binding
insulin receptor binding
receptor tyrosine kinase binding
phosphate ion binding
phosphoprotein phosphatase activity
hydrolase activity
phosphatase activity
protein phosphatase
protein modifying enzyme
cell adhesion
transmembrane receptor protein tyrosine phosphatase signaling pathway
chemical synaptic transmission
cell migration
neuron projection regeneration
peptidyl-tyrosine dephosphorylation
regulation of axon regeneration
negative regulation of receptor binding
negative regulation of cytokine-mediated signaling pathway
protein dephosphorylation
homophilic cell adhesion via plasma membrane adhesion molecules
nervous system development
negative regulation of cell proliferation
regulation of neuron projection development
negative regulation of cell projection organization
negative regulation of epidermal growth factor receptor signaling pathway
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
negative regulation of insulin receptor signaling pathway
positive regulation of dendrite morphogenesis
regulation of synapse structure or activity
negative regulation of neurotrophin TRK receptor signaling pathway
signal transduction
dephosphorylation
