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HSF1 Antibody (MA5-45713) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with HSF1 monoclonal antibody (Product # MA5-45713) at 1:100 for 12 hours at 4°C, followed by R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 antibody. (C... View More
产品信息
MA5-45713
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
宿主/亚型
Rat
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4B4
抗原
Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
内含物
no preservative
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932167
产品详细信息
1 µg/mL of MA5-45713 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.|Detects approximately 85kDa (unstressed cell lysates) and approximately 95kDa (heat shocked cell lysates).
靶标信息
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: heat shock factor 1; heat shock factor 2; Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF 1; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1
基因别名: AA960185; HSF; HSF1; Hsf1alpha; Hsf1beta; HSTF1
UniProt ID: (Bovine) Q08DJ8, (Human) Q00613, (Mouse) P38532
DNA binding
heat shock protein binding
chromatin DNA binding
identical protein binding
sequence-specific DNA binding
protein self-association
protein heterodimerization activity
Hsp90 protein binding
translation elongation factor binding
promoter-specific chromatin binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
DNA repair
mRNA processing
positive regulation of cell proliferation
negative regulation of protein complex assembly
cellular response to heat
cellular response to unfolded protein
cellular macromolecular complex assembly
positive regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
mRNA transport
positive regulation of transcription from RNA polymerase II promoter in response to heat stress
cellular response to cadmium ion
cellular response to copper ion
cellular response to gamma radiation
cellular response to diamide
regulation of cellular response to heat
positive regulation of mRNA polyadenylation
cellular response to sodium arsenite
negative regulation of double-strand break repair via nonhomologous end joining
embryonic placenta development
protein phosphorylation
defense response
female meiotic division
spermatogenesis
negative regulation of cell proliferation
mRNA transcription
response to lipopolysaccharide
negative regulation of tumor necrosis factor production
positive regulation of multicellular organism growth
embryonic process involved in female pregnancy
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
response to heat
transcription from RNA polymerase II promoter
regulation of spindle checkpoint
