Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
4-Hydroxynonenal Antibody (MA5-45789) in WB
Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 µg). Lane 4: 4-hydroxy nonenal-BSA (2.0 µg). Lane 5: 4-hydroxy-2-hexenal (0.5 µg). Lane 6: 4-hydroxy-2-hexenal (2.0 µg). Blocking: 5% Skim Milk in TBST. Samples were incubated with 4-hydroxy-nonenal monoclonal antibody (Product # MA5-45789) at 1:1,000 for 2 hours at RT, followed by Goat Anti-Mouse IgG: HRP at 1:2,000 for 60 min at RT. ... View More
产品信息
MA5-45789
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
12F7
抗原
Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932243
产品详细信息
A 1:1,000 dilution of MA5-45789 was sufficient for detection of 4-Hydroxynonenal in 0.5 µg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
靶标信息
4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, is an alpha, beta-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha, beta-unsaturated hydroxyalkenal formed in this process.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: 4-HNE; 4-hydroxy Hexenal; 4-Hydroxy Nonenal; 4-hydroxy-2-nonenal; 4-Hydroxynonenal; 4HNE; HNE
