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MHC Class II Antibody (MA5-46188) in Flow
Flow Cytometry analysis of MHC Class II using MHC Class II Monoclonal Antibody (M5/114), Biotin (Product # MA5-46188). Separation of murine MHC Class II positive splenocytes (red-filled) from MHC Class II negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-mouse MHC Class II (M5/114) Biotin antibody (concentration in sample 9 μg/mL, Streptavidin APC).
产品信息
MA5-46188
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
M5/114
抗原
Activated C57BL/6 mouse spleen cells
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Size-exclusion chromatography
保存液
PBS, pH 7.4, with 0.2% BSA
内含物
15mM sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2937581
产品详细信息
This antibody recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
靶标信息
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MHC 2; MHC Class II; MHC II; MHCCII
