Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
MAL Antibody (PA1-085) in WB
Western blot analysis of MAL was performed by loading 50 µg of mouse mpkCCD and Xenopus 2f3 whole cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% nonfat dry milk in TBS for 1 hour at room temperature. The membrane was probed with a MAL polyclonal antibody (Product # PA1-085) at a dilution of 1:500 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody for 1 hour. Detection was performed using SuperSignal West Dura (Product # 34075). Data ... View More
产品信息
PA1-085
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat,
Xenopus
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptides corresponding to residues C(114)F D G F T Y (R/K) H Y H E N I(126) of human MAL.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2139001
产品详细信息
PA1-085 detects MAL from rat, mouse, xenopus and human samples.
PA1-085 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~ 17 kDa protein corresponding to MAL from rat kidney extracts.
The PA1-085 immunogen is a synthetic peptides corresponding to residues C(114)F D G F T Y (R/K) H Y H E N I(126) of human MAL. This peptide (Cat. # PEP-249) can be used in neutralization and control experiments.
靶标信息
MAL, a nonglycosylated integral membrane proteolipid, has been identified as a major internal component of glycolipid-enriched membrane (GEM) microdomains. MAL is the first integral membrane proteolipid component to have been shown as a necessary element in the cellular apical sorting apparatus for newly synthesized proteins. In its steady state this highly hydrophobic proteolipid, resides predominantly in the apical zone of polarized epithelial cells. It has also been shown in itinerant protein cycling between the plasma membrane and endosomes and in other cells. This 17-kDa proteolipid contains four putative transmembrane domains. These domains along with its protein trafficking properties are defined by the specific signals delegated by its specific amino acid sequence.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 17 kDa myelin vesicular protein; mal T-cell differentiation protein; MVP17; MyD88-adapter-like; Myelin and lymphocyte protein; myelin and lymphocyte protein, T-cell differentiation protein; NS 3; T-lymphocyte maturation-associated protein
基因别名: MAL; MALGENE; Mpv17; Mvp17; Vip17; XELAEV_18026637mg
UniProt ID: (Human) P21145, (Mouse) O09198, (Rat) Q64349
Entrez Gene ID: (Human) 4118, (Mouse) 17153, (Rat) 25263, (Xenopus) 379738
