Invitrogen

MATR3 Recombinant Rabbit Monoclonal Antibody (JU93-43)

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

查看其他11个MATR3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (14)
高级验证 (4)

MATR3 Antibody in Immunocytochemistry (ICC/IF)

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MATR3 Antibody (MA5-34642) in ICC/IF

Immunofluorescence of matrin-3 was performed using HAP1 WT and MATR3 KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cells were stain... View More

MATR3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MATR3 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal in immunofluorescence was observed for target protein in MATR3 knockout (KO) HAP1 cell line using Anti-MATR3 recombinant monoclonal antibody (Product # MA5-34642). Parental cells were transfected with CellTracker Green (Product # C2925) and MATR3 KO with CellTracker Deep Red (Product # C34565). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation. {KO}

产品信息

MA5-34642

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:1,000

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JU93-43

抗原

Synthetic peptide within Human Matrin 3 aa 749-798

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Wet ice

RRID

AB_2848550

产品详细信息

Positive Control: Daudi, mouse brain tissue lysate, Hela, LOVO, SH-SY-5Y, rat brain tissue, human tonsil tissue, human lung cancer tissue, human liver tissue, mouse testis tissue.

靶标信息

This gene encodes a nuclear matrix protein, which is proposed to stabilize certain messenger RNA species. Mutations of this gene are associated with distal myopathy 2, which often includes vocal cord and pharyngeal weakness. Alternatively spliced transcript variants, including read-through transcripts composed of the upstream small nucleolar RNA host gene 4 (non-protein coding) and matrin 3 gene sequence, have been identified. Pseudogenes of this gene are located on chromosomes 1 and X.

仅用于科研。不用于诊断过程。未经明确授权不得转售。