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MBD2 Antibody (MA5-42844) in WB
Western blot analysis of MBD2 in extracts of Mouse liver cells. Samples were incubated with MBD2 Monoclonal antibody (Product # MA5-42844) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
产品信息
MA5-42844
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
4D4D5
抗原
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MBD2 (Q9UBB5).
偶联物
Unconjugated
形式
Liquid
浓度
2.18 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2911985
产品详细信息
Positive test controls include: Mouse liver.
Immunogen sequence: GGSGLGGDGG GCGGGGSGGG GAPRREPVPF PSGSAGPGPR GPRATESGKR MDCPALPPGW KKEEVIRKSG LSAGKSDVYY FSPSGKKFRS KPQLARYLGN T
靶标信息
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp586O0821; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2
基因别名: Mbd2; MBD2a
UniProt ID: (Mouse) Q9Z2E1
Entrez Gene ID: (Mouse) 17191
