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Invitrogen

CD45 Monoclonal Antibody (30-F11), PE-Texas Red

4个已发表图片

15 篇参考文献

查看其他310个CD45抗体

说明书

实验方案

技术支持

货号 MCD4517

CNY 5,813.00

500 µL

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说明书

实验方案

技术支持

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CD45 Antibody (MCD4517) in Flow

Cell research 2020 - Fig. 1 Derivation of IMRCs from hESCs. a Different phase of the IMRCs derivation protocol. b Representative morphology of cells at different stages as observed by phase contrast microscopy. hEBs human embryoid bodies. Scale bar, 100 um. c A representative chromosome spread of normal diploid IMRCs with 22 pairs of autosomes and two X chromosomes. d Copy number variation (CNV) analysis by whole-genome sequenc... View More

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产品信息

MCD4517

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-dependent

查看15篇已发表文章 15篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

30-F11

抗原

The CD45 antigen

偶联物

PE-Texas Red

激发/发射光谱

567/615 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10392557

产品详细信息

The Rat anti-Mouse CD45 Monoclonal Antibody (mAb) recognizes all isoforms of the CD45 antigen. Applications reported for this antibody include flow cytometry, immunohistochemistry (zinc- and formalin-fixed, paraffin embedded), and immunohistochemistry (acetone-fixed, frozen). For this PE-Texas Red™ conjugate: excitation 488 nm/peak emission 616 (nm).

If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Cells should be analyzed within 18 hours of staining for best results. Avoid light exposure with fluorochrome-conjugated antibodies. Use dim light during handling, incubation with cells, and prior to analysis.

靶标信息

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder

The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.

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Product References

Autotaxin Inhibition with IOA-289 Decreases Breast Tumor Growth in Mice Whereas Knockout of Autotaxin in Adipocytes Does Not.

Cancers

Tang X,Morris AJ,Deken MA,Brindley DN

MCD4517 was used in Flow cytometry/Cell sorting to propose that inhibiting autotaxin activity that is derived from cells presenting breast tumors such as fibroblasts, leukocytes, or endothelial cells changes the tumor micro-environment in such a way as to inhibit tumor growth.

Fri May 26 00:00:00 UTC 2023

Hypereosinophilia causes progressive cardiac pathologies in mice.

iScience

Diny NL,Wood MK,Won T,Talor MV,Lukban C,Bedja D,Wang N,Kalinoski H,Daoud A,Talbot CC,Leei Lin B,Čiháková D

MCD4517 was used in Flow Cytometry to investigate the mechanisms of eosinophil-mediated tissue damage.

Fri Oct 20 00:00:00 UTC 2023

RIPK3 and caspase 8 collaborate to limit herpes simplex encephalitis.

PLoS pathogens

Guo H,Koehler HS,Mocarski ES,Dix RD

MCD4517 was used in Flow cytometry/Cell sorting to show that kinase-independent induction of inflammation by RIPK3 in collaboration with caspase 8 restricts virus replication within the brain during HSE neuropathogenesis.

Thu Sep 01 00:00:00 UTC 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

MCD4517 was used in Flow Cytometry to pave the way for LSC-directed disease monitoring and therapy in this disease.

Mon Feb 01 00:00:00 UTC 2021

Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis.

Cell research

Wu J,Song D,Li Z,Guo B,Xiao Y,Liu W,Liang L,Feng C,Gao T,Chen Y,Li Y,Wang Z,Wen J,Yang S,Liu P,Wang L,Wang Y,Peng L,Stacey GN,Hu Z,Feng G,Li W,Huo Y,Jin R,Shyh-Chang N,Zhou Q,Wang L,Hu B,Dai H,Hao J

Published figure using CD45 monoclonal antibody (Product # MCD4517) in Flow Cytometry

Tue Sep 01 00:00:00 UTC 2020

Yap1-Driven Intestinal Repair Is Controlled by Group 3 Innate Lymphoid Cells.

Cell reports

Romera-Hernández M,Aparicio-Domingo P,Papazian N,Karrich JJ,Cornelissen F,Hoogenboezem RM,Samsom JN,Cupedo T

MCD4517 was used in Flow cytometry/Cell sorting to investigate the mechanisms of ILC3-driven tissue regeneration.

Tue Jan 07 00:00:00 UTC 2020

The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis.

Cell reports

Hou X,Chen G,Bracamonte-Baran W,Choi HS,Diny NL,Sung J,Hughes D,Won T,Wood MK,Talor MV,Hackam DJ,Klingel K,Davogustto G,Taegtmeyer H,Coppens I,Barin JG,Čiháková D

Published figure using CD45 monoclonal antibody (Product # MCD4517) in Flow Cytometry

Tue Jul 02 00:00:00 UTC 2019

CX3CL1-Fc treatment prevents atherosclerosis in Ldlr KO mice.

Molecular metabolism

Riopel M,Vassallo M,Ehinger E,Pattison J,Bowden K,Winkels H,Wilson M,de Jong R,Patel S,Balakrishna D,Bilakovics J,Fanjul A,Plonowski A,Larson CJ,Ley K,Cabrales P,Witztum JL,Olefsky JM,Lee YS

MCD4517 was used in fluorescence-activated cell sorting to indicate that CX3CL1-Fc can inhibit monocyte/endothelial cell adhesion as well as reduce atherosclerosis.

Fri Feb 01 00:00:00 UTC 2019

MyD88 signaling in dendritic cells and the intestinal epithelium controls immunity against intestinal infection with C. rodentium.

PLoS pathogens

Friedrich C,Mamareli P,Thiemann S,Kruse F,Wang Z,Holzmann B,Strowig T,Sparwasser T,Lochner M

MCD4517 was used in Flow cytometry/Cell sorting to suggest that MyD88 signaling in DC and IEC is both essential and sufficient to induce a full spectrum of host responses upon intestinal infection with C. rodentium.

Mon May 01 00:00:00 UTC 2017

Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.

Nature communications

Douam F,Hrebikova G,Albrecht YE,Sellau J,Sharon Y,Ding Q,Ploss A

MCD4517 was used in Flow cytometry/Cell sorting to demonstrate the tracking of and sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models.

Tue Mar 14 00:00:00 UTC 2017

A depleting antibody toward sca-1 mitigates a surge of CD34(+)/c-kit(+) progenitors and reduces vascular restenosis in a murine vascular injury model.

Journal of vascular surgery

Tillman BW,Kelly J,Richards TD,Chen AF,Donnenberg AD,Donnenberg VS,Tzeng E

MCD4517 was used in flow cytometry to determine if circulating CD34(+)/c-kit(+) progenitors increase after vascular injury and correlate with stromal cell-derived factor 1 levels

Sat Oct 01 00:00:00 UTC 2016

Interleukin-17-producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity.

Nature medicine

Kim HY,Lee HJ,Chang YJ,Pichavant M,Shore SA,Fitzgerald KA,Iwakura Y,Israel E,Bolger K,Faul J,DeKruyff RH,Umetsu DT

MCD4517 was used in flow cytometry to elucidate the immunological pathways that lead to obesity-associated asthma.

Wed Jan 01 00:00:00 UTC 2014

HMGB1 enhances immune suppression by facilitating the differentiation and suppressive activity of myeloid-derived suppressor cells.

Cancer research

Parker KH,Sinha P,Horn LA,Clements VK,Yang H,Li J,Tracey KJ,Ostrand-Rosenberg S

Published figure using CD45 monoclonal antibody (Product # MCD4517) in Flow Cytometry

Wed Oct 15 00:00:00 UTC 2014

Innate lymphoid cells mediate influenza-induced airway hyper-reactivity independently of adaptive immunity.

Nature immunology

Chang YJ,Kim HY,Albacker LA,Baumgarth N,McKenzie AN,Smith DE,Dekruyff RH,Umetsu DT

MCD4517 was used in flow cytometry to examine how influenza infection induced airway hyper-reactivity.

Sun May 29 00:00:00 UTC 2011

T-cell regulation of neutrophil infiltrate at the early stages of a murine colitis model.

Inflammatory bowel diseases

van Lierop PP,de Haar C,Lindenbergh-Kortleve DJ,Simons-Oosterhuis Y,van Rijt LS,Lambrecht BN,Escher JC,Samsom JN,Nieuwenhuis EE

MCD4517 was used in flow cytometry to examine the effect of tacrolimus in a murine colitis model

Mon Mar 01 00:00:00 UTC 2010

生物信息学

蛋白别名: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220

基因别名: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

UniProt ID: (Mouse) P06800

Entrez Gene ID: (Mouse) 19264

功能

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

参与通路

CD45 Monoclonal Antibody (30-F11), PE-Texas Red

CD45 Antibody (MCD4517) in Flow

产品信息

MCD4517

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

激发/发射光谱

形式

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!

CD45 Monoclonal Antibody (30-F11), PE-Texas Red

CD45 Antibody (MCD4517) in Flow

产品信息

MCD4517

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

激发/发射光谱

形式

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID