Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 4
MCP-1 Antibody (700489) in ICC/IF
Immunofluorescence analysis of MCP-1 was performed using 70% confluent log phase U-937 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MCP-1 Recombinant Rabbit Monoclonal Antibody (29H86L56) (Product # 700489) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus ... View More
产品信息
700489
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
29H86L56
抗原
proprietary
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532326
产品详细信息
This antibody is predicted to react with Rhesus monkey and porcine based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; RP23-350G1.3; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2
基因别名: CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
UniProt ID: (Human) P13500
Entrez Gene ID: (Human) 6347
MAPK cascade
angiogenesis
response to hypoxia
positive regulation of leukocyte mediated cytotoxicity
positive regulation of endothelial cell proliferation
leukocyte migration involved in inflammatory response
monocyte chemotaxis
positive regulation of cellular extravasation
protein phosphorylation
cellular calcium ion homeostasis
chemotaxis
inflammatory response
humoral immune response
cytoskeleton organization
cell adhesion
signal transduction
cell surface receptor signaling pathway
transforming growth factor beta receptor signaling pathway
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
JAK-STAT cascade
aging
regulation of cell shape
response to heat
response to wounding
response to mechanical stimulus
response to bacterium
organ morphogenesis
response to gamma radiation
regulation of vascular endothelial growth factor production
positive regulation of macrophage chemotaxis
response to activity
negative regulation of angiogenesis
viral genome replication
cytokine-mediated signaling pathway
cellular homeostasis
neutrophil chemotaxis
organ regeneration
lipopolysaccharide-mediated signaling pathway
response to progesterone
positive regulation of tumor necrosis factor production
cellular response to insulin stimulus
positive regulation of collagen biosynthetic process
response to vitamin B3
negative regulation of glial cell apoptotic process
helper T cell extravasation
cellular response to drug
cellular response to macrophage colony-stimulating factor stimulus
cellular response to platelet-derived growth factor stimulus
PERK-mediated unfolded protein response
response to amino acid
protein kinase B signaling
negative regulation of neuron apoptotic process
positive regulation of GTPase activity
astrocyte cell migration
cellular response to fibroblast growth factor stimulus
response to ethanol
response to antibiotic
vascular endothelial growth factor receptor signaling pathway
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of inflammatory response
positive regulation of synaptic transmission
positive regulation of T cell activation
positive regulation of nitric-oxide synthase biosynthetic process
maternal process involved in female pregnancy
maternal process involved in parturition
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to retinoic acid
cellular response to ATP
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to high density lipoprotein particle stimulus
cellular response to organic cyclic compound
cellular response to dexamethasone stimulus
positive regulation of monocyte chemotaxis
positive regulation of immune complex clearance by monocytes and macrophages
positive regulation of calcium ion import
positive regulation of protein targeting to membrane
positive regulation of apoptotic cell clearance
negative regulation of natural killer cell chemotaxis
