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MCP-2 Antibody (500-M69-500UG) in WB
Western Blot: To detect Human MCP-2 (CCL8) by Western Blot analysis MCP-2 Monoclonal Antibody (Product # 500-M69-500UG) can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-2 (CCL8) is 0.25-0.50 ng/lane, under non-reducing conditions.
产品信息
500-M69-500UG
产品规格
种属反应
Human
宿主/亚型
Mouse
分类
Monoclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human MCP-2 (CCL8)
偶联物
形式
Lyophilized
纯化类型
Protein G
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2942205
产品详细信息
AA Sequence of recombinant protein: QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP.
Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human MCP-2 (CCL8). Anti-Human MCP-2 (CCL8)-specific antibody was purified from cell culture by Protein G affinity chromatography.
Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 500 pg/mL of Recombinant Human MCP-2 (CCL8) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-2 (CCL8) (500-P35BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/mL.
Western Blot: To detect Human MCP-2 (CCL8) by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-2 (CCL8) is 0.25-0.50 ng/lane, under non-reducing conditions.
靶标信息
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-C motif chemokine 8; chemokine (C-C motif) ligand 8; HC14; MCP-2; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); Small-inducible cytokine A8
基因别名: CCL8; HC14; MCP-2; MCP2; SCYA10; SCYA8
UniProt ID: (Human) P80075
Entrez Gene ID: (Human) 6355
monocyte chemotaxis
positive regulation of leukocyte migration
protein phosphorylation
calcium ion transport
cellular calcium ion homeostasis
exocytosis
chemotaxis
inflammatory response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
response to virus
neutrophil chemotaxis
positive regulation of GTPase activity
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
negative regulation of leukocyte proliferation
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
