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MCP-2 Antibody (500-P127BT-25UG) in WB
Western Blot: To detect mMCP-2 by Western Blot analysis MCP-2 Polyclonal Antibody, Biotin (Product # 500-P127BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P127BT-25UG
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine MCP-2 (CCL8)
偶联物
Biotin
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929370
产品详细信息
AA Sequence of recombinant protein: GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVV FQTKQGMSLC VDPTQKWVSE YMEILDQKSQ ILQP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MCP-2 (CCL8). Anti-Murine MCP-2 (CCL8)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine MCP-2 (CCL8) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MCP-2 (CCL8) (500-P127) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MCP-2 (CCL8).
Western Blot: To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P127BT-1MG will be provided as 2 x 500 µg
靶标信息
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-C motif chemokine 8; MCP-2; Monocyte chemoattractant protein 2; monocyte chemoattractant protein-2; Monocyte chemotactic protein 2; small inducible cytokine A8; Small-inducible cytokine A8
基因别名: 1810063B20Rik; AB023418; Ccl8; HC14; MCP-2; Mcp2; Scya8
UniProt ID: (Mouse) Q9Z121
Entrez Gene ID: (Mouse) 20307
