Invitrogen

MCT1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1个已发表图片

1 篇参考文献

查看其他20个MCT1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

MCT1 Antibody in Immunocytochemistry (ICC/IF)

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MCT1 Antibody (PA5-78169) in ICC/IF

Immunofluorescence analysis of MCT1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MCT1 Polyclonal Antibody (Product # PA5-78169) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32790... View More

MCT1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

MCT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with MCT1 siRNA and reduction of signal was observed in Western Blot using MCT1 Polyclonal Antibody (Product # PA5-78169). {KD}

产品信息

PA5-78169

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

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免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human MCT1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2736129

产品详细信息

Positive Control: 293T, A431, HeLa, HepG2, human MCT1-transfected 293T

Predicted Reactivity: Rhesus Monkey (96%), Chimpanzee (99%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SLC16A1 is a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes. Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Targeting Lactate Metabolism by Inhibiting MCT1 or MCT4 Impairs Leukemic Cell Proliferation, Induces Two Different Related Death-Pathways and Increases Chemotherapeutic Sensitivity of Acute Myeloid Leukemia Cells.

Frontiers in oncology

Saulle E,Spinello I,Quaranta MT,Pasquini L,Pelosi E,Iorio E,Castelli G,Chirico M,Pisanu ME,Ottone T,Voso MT,Testa U,Labbaye C

PA5-78169 was used in Western Blotting to show that MCT1 and MCT4 play a key role in leukemic cell proliferation and suggest that lactate metabolism is a potential target for AML therapy.

Tue Feb 23 00:00:00 EST 2021

Frontiers in oncology

Saulle E,Spinello I,Quaranta MT,Pasquini L,Pelosi E,Iorio E,Castelli G,Chirico M,Pisanu ME,Ottone T,Voso MT,Testa U,Labbaye C

PA5-78169 was used in Western Blotting to show that MCT1 and MCT4 play a key role in leukemic cell proliferation and suggest that lactate metabolism is a potential target for AML therapy.

Tue Feb 23 00:00:00 EST 2021

生物信息学

蛋白别名: FLJ36745; MCT 1; MGC44475; monocarboxylate transporter; Monocarboxylate transporter 1; SLC16A1 protein; solute carrier family 16 (monocarboxylate transporter), member 1; solute carrier family 16 (monocarboxylic acid transporters), member 1; Solute carrier family 16 member 1; solute carrier family 16, member 1 (monocarboxylic acid transporter 1)

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基因别名: HHF7; MCT; MCT1; MCT1D; SLC16A1

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UniProt ID: (Human) P53985

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Entrez Gene ID: (Human) 6566

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功能

monocarboxylic acid transmembrane transporter activity lactate transmembrane transporter activity mevalonate transmembrane transporter activity symporter activity protein homodimerization activity organic cyclic compound binding transporter

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