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MDH2 Antibody (PA5-89278) in WB
Western blot analysis of MDH2 in extracts of various cell lines. Samples were incubated with MDH2 Polyclonal antibody (Product # PA5-89278) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
产品信息
PA5-89278
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 59-338 of human MDH2 (NP_0059092)
偶联物
Unconjugated
形式
Liquid
浓度
0.42 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2805463
产品详细信息
Immunogen sequence: AHTPGVAADL SHIETKAAVK GYLGPEQLPD CLKGCDVVVI PAGVPRKPGM TRDDLFNTNA TIVATLTAAC AQHCPEAMIC VIANPVNSTI PITAEVFKKH GVYNPNKIFG VTTLDIVRAN TFVAELKGLD PARVNVPVIG GHAGKTIIPL ISQCTPKVDF PQDQLTALTG RIQEAGTEVV KAKAGAGSAT LSMAYAGARF VFSLVDAMNG KEGVVECSFV KSQETECTYF STPLLLGKKG IEKNLGIGKV SSFEEKMISD AIPELKASIK KGEDFVKTLK; Positive Samples: 293T, HepG2, Jurkat, A-549, MCF7, HeLa, Mouse kidney, Mouse heart, Mouse skeletal muscle; Cellular Location: Mitochondrion matrix
靶标信息
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: malate dehydrogenase 2, NAD (mitochondrial); Malate dehydrogenase, mitochondrial; mitochondrial malate dehydrogenase 2, NAD; testicular tissue protein Li 120
基因别名: M-MDH; MDH; Mdh-2; MDH2; MGC:3559; Mor-1; MOR1
UniProt ID: (Human) P40926, (Mouse) P08249
Entrez Gene ID: (Human) 4191, (Mouse) 17448
