Invitrogen

MEK1 Monoclonal Antibody (3D9)

高级验证

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

3 篇参考文献

查看其他81个MEK1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (6)
高级验证 (2)

图: 1 / 8

MEK1 Antibody (13-3500) in WB

Knockout of MEK1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR934388_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of MEK1 was performed by loading 30 µg of HeLa Cas9 (Lane 1), andHeLa MEK1 KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting Syst... View More

MEK1 Antibody in Immunocytochemistry (ICC/IF)

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MEK1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inMEK1 KO cell line compared to control cell line using Anti-MEK1 Monoclonal Antibody (3D9) (Product # 13-3500). {KO}

产品信息

13-3500

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

2-4 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

2-5 µg

产品规格

种属反应

Dog, Human, Mouse, Non-human primate, Rat, Xenopus

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3D9

抗原

Purified MEK from human T cells and recombinant MEK1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533012

靶标信息

MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PTPIP51—A New RelA-tionship with the NFκB Signaling Pathway.

Biomolecules

Brobeil A,Kämmerer F,Tag C,Steger K,Gattenlöhner S,Wimmer M

13-3500 was used in proximity ligation assay to elucidate how protein tyrosine phosphatase interacting protein 51 modulates the NF-kappaB signaling pathway.

Thu Apr 16 00:00:00 EDT 2015

Expression of Galpha 13 (Q226L) induces P19 stem cells to primitive endoderm via MEKK1, 2, or 4.

The Journal of biological chemistry

Wang HY,Kanungo J,Malbon CC

Fri Feb 01 00:00:00 EST 2002

An analysis of Mek1 signaling in cell proliferation and transformation.

The Journal of biological chemistry

Greulich H,Erikson RL

Fri May 22 00:00:00 EDT 1998

生物信息学

蛋白别名: dual specificity mitogen activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAP kinase kinase or Erk Kinase, Dual specificity mitogen-activated protein kinase kinase, involved in ras mediated vulval induction, LEThal LET-537 (42.8 kD) (mek-2); MAP kinase/Erk kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; MEK1; mitogen activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; MKK (Thr292); MKK (Thr386); MKK2; MP2K1; PRKMK2; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

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基因别名: CFC3; MAP2K1; MAPKK1; mek-2; MEK1; MEKK1; MKK1; PRKMK1; XELAEV_18018447mg