Invitrogen

CD3 Monoclonal Antibody (7D6), PE-Cyanine7

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CD3 Antibody (MHCD0312) in Flow

Human peripheral blood lymphocytes stained using PE-Cy®7 of anti-human CD3 monoclonal antibody (clone S4.1). The negative control profiles represent unstained cells.

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产品信息

MHCD0312

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

7D6

抗原

Human CD3 epsilon

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA, 10% sucrose

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10373692

产品详细信息

The R-phycoerythrin (PE)-Cy7® tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a 488 nm laser without occupying traditional channels associated with FITC and PE.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

Analyte Specific Reagent

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Product References

Distinct mucosal and systemic immunological characteristics in transgender women potentially relating to HIV acquisition.

JCI insight

Schuetz A,Corley MJ,Sacdalan C,Phuang-Ngern Y,Nakpor T,Wansom T,Ehrenberg PK,Sriplienchan S,Thomas R,Ratnaratorn N,Sukhumvittaya S,Tragonlugsana N,Slike BM,Akapirat S,Pinyakorn S,Rerknimitr R,Pang AP,Kroon E,Teeratakulpisan N,Krebs SJ,Phanuphak N,Ndhlovu LC,Vasan S

MHCD0312 was used in Flow cytometry/Cell sorting to show that it is possible that increased inflammation and higher frequencies of CCR5-expressing target cells at sites of mucosal viral entry may contribute to increased risk of HIV acquisition in TGW, with further validation in larger studies warranted.

Tue Aug 22 00:00:00 EDT 2023

A new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease

Grahmann PR,Braun RK

MHCD0312 was used in flow cytometry to demonstrate that aerosolized interferon-gamma results in the transient conversion of sputum smears in multidrug-resistant pulmonary tuberculosis

Sun Jun 01 00:00:00 EDT 2008

Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases.

Cytometry. Part B, Clinical cytometry

Braun RK,Foerster M,Grahmann PR,Haefner D,Workalemahu G,Kroegel C

MHCD0312 was used in flow cytometry to test if CD103 has a regulatory role in the microenvironment of the epithelial cell layer.

Tue Jul 01 00:00:00 EDT 2003

生物信息学

蛋白别名: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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基因别名: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ