Invitrogen

TCR gamma/delta Monoclonal Antibody (5A6.E9), PE

产品信息

MHGD04

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

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产品规格

种属反应

Human

已发表种属

Human, Rhesus monkey

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

5A6.E9

抗原

Human TCR gamma/delta T cell receptor

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA, sucrose

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10374518

产品详细信息

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

靶标信息

TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Cell reports. Medicine

Rouers A,Chng MHY,Lee B,Rajapakse MP,Kaur K,Toh YX,Sathiakumar D,Loy T,Thein TL,Lim VWX,Singhal A,Yeo TW,Leo YS,Vora KA,Casimiro D,Lim B,Tucker-Kellogg L,Rivino L,Newell EW,Fink K

MHGD04 was used in Flow Cytometry to reveal associations between cellular profiles and disease severity, opening opportunities to study immunopathology in dengue disease and the potential predictive value of these parameters.

Tue May 18 00:00:00 EDT 2021

Fc-mediated effector function contributes to the in vivo antiviral effect of an HIV neutralizing antibody.

Proceedings of the National Academy of Sciences of the United States of America

Asokan M,Dias J,Liu C,Maximova A,Ernste K,Pegu A,McKee K,Shi W,Chen X,Almasri C,Promsote W,Ambrozak DR,Gama L,Hu J,Douek DC,Todd JP,Lifson JD,Fourati S,Sekaly RP,Crowley AR,Ackerman ME,Ko SH,Kilam D,Boritz EA,Liao LE,Best K,Perelson AS,Mascola JR,Koup RA

MHGD04 was used in Flow cytometry/Cell sorting to test the role of effector function in vivo by comparing the rate and timing of plasma-virus clearance in response to a single-dose treatment with either unmodified NAb or those with either reduced or augmented Fc function.

Tue Aug 04 00:00:00 EDT 2020

Chronic Inflammation Permanently Reshapes Tissue-Resident Immunity in Celiac Disease.

Cell

Mayassi T,Ladell K,Gudjonson H,McLaren JE,Shaw DG,Tran MT,Rokicka JJ,Lawrence I,Grenier JC,van Unen V,Ciszewski C,Dimaano M,Sayegh HE,Kumar V,Wijmenga C,Green PHR,Gokhale R,Jericho H,Semrad CE,Guandalini S,Dinner AR,Kupfer SS,Reid HH,Barreiro LB,Rossjohn J,Price DA,Jabri B

MHGD04 was used in Flow cytometry/Cell sorting to determine the role of tissue-resident lymphocytes in chronic inflammation.

Thu Feb 21 00:00:00 EST 2019

Mapping the Lineage Relationship between CXCR5⁺ and CXCR5^- CD4⁺ T Cells in HIV-Infected Human Lymph Nodes.

Cell reports

Del Alcazar D,Wang Y,He C,Wendel BS,Del Río-Estrada PM,Lin J,Ablanedo-Terrazas Y,Malone MJ,Hernandez SM,Frank I,Naji A,Reyes-Terán G,Jiang N,Su LF

MHGD04 was used in Flow cytometry/Cell sorting to link lymph node (LN) pathology to circulating T cells and expand the current understanding on the diversity of T cells that regulate B cell responses during chronic inflammation.

Tue Sep 17 00:00:00 EDT 2019

A Macrophage Colony-Stimulating-Factor-Producing γδ T Cell Subset Prevents Malarial Parasitemic Recurrence.

Immunity

Mamedov MR,Scholzen A,Nair RV,Cumnock K,Kenkel JA,Oliveira JHM,Trujillo DL,Saligrama N,Zhang Y,Rubelt F,Schneider DS,Chien YH,Sauerwein RW,Davis MM

MHGD04 was used in Flow Cytometry to point to an M-CSF-producing γδ T cell subset that fulfills a specialized protective role in the later stage of malaria infection when αβ T cells have declined.

Tue Feb 20 00:00:00 EST 2018

Mass cytometry analysis of human T cell phenotype and function.

Methods in molecular biology (Clifton, N.J.)

Newell EW,Yun LL

MHGD04 was used in flow cytometry to describe methods of using mass cytometery for the analysis of human T cell surface markers and intracellular cytokines.

Thu Apr 16 00:00:00 EDT 2015

Interleukin 17-producing gamma delta T cells increased in patients with active pulmonary tuberculosis.

Cellular & molecular immunology

Peng MY,Wang ZH,Yao CY,Jiang LN,Jin QL,Wang J,Li BQ

MHGD04 was used in Flow Cytometry to elucidate the mechanisms by which gammadelta T cells participate in the innate and/or acquired immunity to tuberculosis.

Sun Jun 01 00:00:00 EDT 2008

Vaccinia virus inhibits T cell receptor-dependent responses by human gammadelta T cells.

The Journal of infectious diseases

Li H,Deetz CO,Zapata JC,Cairo C,Hebbeler AM,Propp N,Salvato MS,Shao Y,Pauza CD

MHGD04 was used in Flow cytometry/Cell sorting to suggest that increased VV replication or expression in the absence of gammadelta T cell responses might contribute to its potency as a vaccine against poxvirus and recombinant antigens.

Mon Jan 01 00:00:00 EST 2007

生物信息学

蛋白别名: T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex

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