Invitrogen

MIF Polyclonal Antibody

MIF Antibody in Immunocytochemistry (ICC/IF)

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MIF Antibody (PA5-27343) in ICC/IF

Immunofluorescent analysis of 14-3-3 zeta (green) HEK293T cells. Cells fixed with 4% formaldehyde fixed were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a 14-3-3 zeta ppolyclonal antibody (Product # PA5-27317) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature... View More

MIF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MIF Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-27343

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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酶联免疫吸附实验 (ELISA)

1:1,000-1:10,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Sheep

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 115 of Human MIF

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544819

产品详细信息

Recommended positive controls: Molt-4, mouse brain.

Predicted reactivity: Mouse (89%), Rat (90%), Pig (94%), Sheep (91%), Rhesus Monkey (100%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction.

Acta biochimica et biophysica Sinica

Guan D,Li Y,Cui Y,Zhao H,Dong N,Wang K,Ren D,Song T,Wang X,Jin S,Gao Y,Wang M

Published figure using MIF polyclonal antibody (Product # PA5-27343) in Western Blot

Mon Jul 10 00:00:00 EDT 2023

Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchyma.

Life science alliance

Yamamoto Y,Kadoya K,Terkawi MA,Endo T,Konno K,Watanabe M,Ichihara S,Hara A,Kaneko K,Iwasaki N,Ishijima M

Published figure using MIF polyclonal antibody (Product # PA5-27343) in Immunohistochemistry

Sat Oct 01 00:00:00 EDT 2022

Macrophage Migration Inhibitory Factor (MIF) and Its Homologue d-Dopachrome Tautomerase (DDT) Inversely Correlate with Inflammation in Discoid Lupus Erythematosus.

Molecules (Basel, Switzerland)

Caltabiano R,De Pasquale R,Piombino E,Campo G,Nicoletti F,Cavalli E,Mangano K,Fagone P

PA5-27343 was used in Immunohistochemistry to evaluate Macrophage migration inhibitory factor in Discoid Lupus Erythematosus lesions and normal skin.

Fri Jan 01 00:00:00 EST 2021

Macrophage Migration Inhibitory Factor (MIF) and Its Homologue d-Dopachrome Tautomerase (DDT) Inversely Correlate with Inflammation in Discoid Lupus Erythematosus.

Molecules (Basel, Switzerland)

Caltabiano R,De Pasquale R,Piombino E,Campo G,Nicoletti F,Cavalli E,Mangano K,Fagone P

PA5-27343 was used in Immunohistochemistry to evaluate Macrophage migration inhibitory factor in Discoid Lupus Erythematosus lesions and normal skin.

Fri Jan 01 00:00:00 EST 2021

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27343 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 EDT 2020

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27343 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 EDT 2020

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27343 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 EDT 2020

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27343 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 EDT 2020

CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing.

Cellular and molecular gastroenterology and hepatology

Farr L,Ghosh S,Jiang N,Watanabe K,Parlak M,Bucala R,Moonah S

Mon May 10 00:00:00 EDT 2021

生物信息学

蛋白别名: Delayed early response protein 6; DER6; GIF; Glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase

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基因别名: GIF; GLIF; MIF; MMIF

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UniProt ID: (Human) P14174, (Mouse) P34884, (Rat) P30904

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Entrez Gene ID: (Human) 4282, (Mouse) 17319, (Rat) 81683

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功能

dopachrome isomerase activity receptor binding cytokine activity cytokine receptor binding protein binding chemoattractant activity phenylpyruvate tautomerase activity isomerase activity protein complex binding decarboxylase

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