Invitrogen

MLH1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

查看其他38个MLH1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (4)
高级验证 (2)

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MLH1 Antibody (PA5-78062) in WB

Western blot was performed using Anti-MLH-1 Rabbit Polyclonal Antibody (Product # PA5-78062) and a 84 kDa band corresponding to MLH-1 was observed in all the cell lines tested except HCT 116 and DU 145 which are reported to be negative. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), SW480 (Lane 3), HCT 116 (Lane 4), PC3 (Lane 5), DU 145 (Lane 6) and Raji (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a ... View More

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MLH1 Antibody — Antibody specificity was demonstrated by relative expression. Western blot analysis using MLH-1 Rabbit Polyclonal Antibody (Product # PA5-78062) showed relative expression in HCT 116 and DU 145, which are null for MLH-1 expression in comparison to other cell lines which express MLH-1. {RE}

产品信息

PA5-78062

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human MLH1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.83 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2736155

产品详细信息

Positive Control: Raji, human MLH1-transfected 293T

Predicted Reactivity: Mouse (95%), Rat (94%), Zebrafish (80%), Xenopus laevis (84%), Pig (95%), Bovine (95%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。