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MLH1 Antibody (MA5-50627) in IHC (P)
Immunohistochemistry of MLH1 in paraffin-embedded human colon carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with MLH1 monoclonal antibody (Product # MA5-50627) with a dilution of 1:1,000 for 1 hour at room t... View More
产品信息
MA5-50627
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
A9F7
抗原
Recombinant protein within human MLH1 aa 452-751/756.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3094007
产品详细信息
Positive control: HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, F9 cell lysate, RAW264.7 cell lysate, C6 cell lysate, A431 cell lysate, Daudi cell lysate, K-562 cell lysate, HL-60 cell lysate, SW480 cell lysate, human colon carcinoma tissue, mouse colon tissue, rat small intestine tissue.
Predicted band size: 85 kDa
Subcellular Location: Nucleus, Chromosome.
靶标信息
MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: colon cancer, nonpolyposis type 2; DNA mismatch repair protein Mlh1; MGC5172; mismatch repair protein 1; MLH-1; mutL homolog 1, colon cancer, nonpolyposis type 2; MutL protein homolog 1
基因别名: 1110035C23Rik; AI317206; AI325952; AI561766; COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MLH1
UniProt ID: (Human) P40692, (Rat) P97679, (Mouse) Q9JK91
Entrez Gene ID: (Human) 4292, (Rat) 81685, (Mouse) 17350
nuclear-transcribed mRNA poly(A) tail shortening
resolution of meiotic recombination intermediates
mismatch repair
double-strand break repair via nonhomologous end joining
male meiosis chromosome segregation
synapsis
spermatogenesis
intrinsic apoptotic signaling pathway in response to DNA damage
female meiosis chromosome segregation
somatic hypermutation of immunoglobulin genes
meiotic metaphase I plate congression
meiotic telomere clustering
isotype switching
negative regulation of mitotic recombination
oogenesis
meiotic spindle midzone assembly
response to hypoxia
somatic recombination of immunoglobulin genes involved in immune response
DNA repair
cellular response to DNA damage stimulus
meiotic nuclear division
reciprocal meiotic recombination
male meiosis
response to toxic substance
somatic recombination of immunoglobulin gene segments
response to drug
meiotic chromosome segregation
homologous chromosome segregation
cellular response to organic cyclic compound
cell cycle
