Invitrogen

MMP2 Polyclonal Antibody

MMP2 Antibody in Immunocytochemistry (ICC/IF)

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MMP2 Antibody (PA5-85197) in ICC/IF

MMP2 Polyclonal Antibody detects MMP2 protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MMP2 stained by MMP2 Polyclonal Antibody (Product # PA5-85197) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .

MMP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MMP2 Antibody in Immunoprecipitation (IP)

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产品信息

PA5-85197

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human MMP2.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.33 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2792342

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (81%), Pig (96%), Rabbit (95%), Chicken (85%), Sheep (95%), Bovine (94%).

Positive Control: PC-3, U87-MG, SK-N-SH, U87-MG conditioned medium, mouse brain, DU145.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

TRIM66 Promotes Malignant Progression of Non-Small-Cell Lung Cancer Cells via Targeting MMP9.

Computational and mathematical methods in medicine

Xu Y,Yang Q,Fang Z,Tan X,Zhang M,Chen W

Published figure using MMP2 polyclonal antibody (Product # PA5-85197) in Western Blot

Tue Aug 02 00:00:00 EDT 2022

Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro.

Molecules (Basel, Switzerland)

Chu Z,Luo Y,Ni T,Zhu M,Feng X,Liu Y,Wang H

PA5-85197 was used in Western Blotting to support that betulonic acid inhibits the invasion and metastasis of gastric cancer cells by mediating cytoskeletal remodeling and regulating epithelial mesenchymal transformation.

Wed Feb 02 00:00:00 EST 2022

Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro.

Molecules (Basel, Switzerland)

Chu Z,Luo Y,Ni T,Zhu M,Feng X,Liu Y,Wang H

Wed Feb 02 00:00:00 EST 2022

Poria Acid, Triterpenoids Extracted from Poria cocos, Inhibits the Invasion and Metastasis of Gastric Cancer Cells.

Molecules (Basel, Switzerland)

Wang H,Luo Y,Chu Z,Ni T,Ou S,Dai X,Zhang X,Liu Y

PA5-85197 was used in Western Blotting to show that Triterpenoids from P. cocos have significant biological activity against gastric cancer, and the mechanism may be involved in the process of epithelial-mesenchymal transformation.

Mon Jun 06 00:00:00 EDT 2022

Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.

International journal of molecular sciences

Alzahrani AM,Rajendran P,Veeraraghavan VP,Hanieh H

PA5-85197 was used in Western Blotting to show that KRL not only activates the IGF-IR-dependent p-PI3K/p-AKT and Nrf2 signaling pathway, but also suppresses caspase-dependent apoptosis.

Tue Mar 23 00:00:00 EDT 2021

Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.

International journal of molecular sciences

Alzahrani AM,Rajendran P,Veeraraghavan VP,Hanieh H

PA5-85197 was used in Western Blotting to show that KRL not only activates the IGF-IR-dependent p-PI3K/p-AKT and Nrf2 signaling pathway, but also suppresses caspase-dependent apoptosis.

Tue Mar 23 00:00:00 EDT 2021

Chondroitin polymerizing factor promotes breast carcinoma cell proliferation, invasion and migration and affects expression of epithelial-mesenchymal transition-related markers.

FEBS open bio

Li Y,Gong H,Feng L,Mao D,Xiao Y,Wang Y,Huang L

PA5-85197 was used in Western Blotting to investigate the role of CHPF in breast carcinoma (BRCA) and its underlying mechanism.

Mon Feb 01 00:00:00 EST 2021

Chondroitin polymerizing factor promotes breast carcinoma cell proliferation, invasion and migration and affects expression of epithelial-mesenchymal transition-related markers.

FEBS open bio

Li Y,Gong H,Feng L,Mao D,Xiao Y,Wang Y,Huang L

PA5-85197 was used in Western Blotting to investigate the role of CHPF in breast carcinoma (BRCA) and its underlying mechanism.

Mon Feb 01 00:00:00 EST 2021

Coronin 1C inhibits melanoma metastasis through regulation of MT1-MMP-containing extracellular vesicle secretion.

Scientific reports

Tagliatela AC,Hempstead SC,Hibshman PS,Hockenberry MA,Brighton HE,Pecot CV,Bear JE

PA5-85197 was used in Western Blotting to combine a conditional knockout of Coronin 1C with a genetically engineered mouse model of PTEN/BRAF-driven melanoma.

Mon Jul 20 00:00:00 EDT 2020

生物信息学

蛋白别名: 72 kDa gelatinase; 72 kDa type IV collagenase; CLG 4A; Collagenase; collagenase type IV-A; Gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP-2; MMPs; neutrophil gelatinase; Progelatinase A; TBE-1; TBE1

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基因别名: CLG4; CLG4A; MMP-2; MMP-II; MMP2; MONA; TBE-1

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UniProt ID: (Human) P08253

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Entrez Gene ID: (Human) 4313

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功能

metalloendopeptidase activity serine-type endopeptidase activity protein binding metallopeptidase activity zinc ion binding metalloprotease

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