Invitrogen

MMP2 Monoclonal Antibody (101)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

17个已发表图片

20 篇参考文献

查看其他31个MMP2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

MMP2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 24

MMP2 Antibody (436000) in ICC/IF

Immunofluorescence analysis of MMP-2/CLG4A was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MMP-2/CLG4A (101) Mouse Monoclonal Antibody (Product # 436000) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More

MMP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MMP2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

MMP2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

MMP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. In Fig a, Relative expression of MMP2 was observed in U-87 MG treated with PTI and HT-1080 treated with PTI in comparison with LNCaP treated with PTI, a cell line that expresses very low levels of MMP2 (https://doi.org/10.1210/en.2002-0157), using Anti-MMP2 Monoclonal Antibody (101) (Product # 436000) in Western Blot. {RE}

产品信息

436000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看10篇已发表文章 10篇已发表文章

免疫组化 (IHC)

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

3-5 µg/1x10^6 cells

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Non-human primate, Rabbit, Rhesus monkey, Rat

已发表种属

Fish, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

101

抗原

Recombinant protein derived from the N-terminus of human MMP-2 protein (accession # P08253, NP_004521 ), which is identical to Rhesus monkey and chimpanzee and 98% similar to rat and rabbit and 97% to mouse.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2532214

靶标信息

MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Elastin stabilization prevents impaired biomechanics in human pulmonary arteries and pulmonary hypertension in rats with left heart disease.

Nature communications

Kucherenko MM,Sang P,Yao J,Gransar T,Dhital S,Grune J,Simmons S,Michalick L,Wulsten D,Thiele M,Shomroni O,Hennig F,Yeter R,Solowjowa N,Salinas G,Duda GN,Falk V,Vyavahare NR,Kuebler WM,Knosalla C

Published figure using MMP2 monoclonal antibody (Product # 436000) in Western Blot

Fri Jul 21 00:00:00 EDT 2023

Elastin stabilization prevents impaired biomechanics in human pulmonary arteries and pulmonary hypertension in rats with left heart disease.

Nature communications

Kucherenko MM,Sang P,Yao J,Gransar T,Dhital S,Grune J,Simmons S,Michalick L,Wulsten D,Thiele M,Shomroni O,Hennig F,Yeter R,Solowjowa N,Salinas G,Duda GN,Falk V,Vyavahare NR,Kuebler WM,Knosalla C

Published figure using MMP2 monoclonal antibody (Product # 436000) in Western Blot

Fri Jul 21 00:00:00 EDT 2023

Elastin stabilization prevents impaired biomechanics in human pulmonary arteries and pulmonary hypertension in rats with left heart disease.

Nature communications

Kucherenko MM,Sang P,Yao J,Gransar T,Dhital S,Grune J,Simmons S,Michalick L,Wulsten D,Thiele M,Shomroni O,Hennig F,Yeter R,Solowjowa N,Salinas G,Duda GN,Falk V,Vyavahare NR,Kuebler WM,Knosalla C

Published figure using MMP2 monoclonal antibody (Product # 436000) in Western Blot

Fri Jul 21 00:00:00 EDT 2023

Knockdown of circ_0113656 assuages oxidized low-density lipoprotein-induced vascular smooth muscle cell injury through the miR-188-3p/IGF2 pathway.

Open medicine (Warsaw, Poland)

Yang M,Luo J,Zhang S,Huang Q,Cao Q

436000 was used in Western Blotting to analyze the RNA expression of circ_0113656, microRNA-188-3p (miR-188-3p), and insulin-like growth factor 2 (IGF2) by quantitative real-time polymerase chain reaction.

Tue Jul 18 00:00:00 EDT 2023

Knockdown of circ_0113656 assuages oxidized low-density lipoprotein-induced vascular smooth muscle cell injury through the miR-188-3p/IGF2 pathway.

Open medicine (Warsaw, Poland)

Yang M,Luo J,Zhang S,Huang Q,Cao Q

Tue Jul 18 00:00:00 EDT 2023

Dietary Evodiamine Inhibits Atherosclerosis-Associated Changes in Vascular Smooth Muscle Cells.

International journal of molecular sciences

Zha Y,Yang Y,Zhou Y,Ye B,Li H,Liang J

436000 was used in Immunohistochemistry and Western Blotting to reveal a role for evodiamine in the regulation of vascular smooth muscle cells in atherosclerosis, highlighting a potential future role for the compound as an anti-atherosclerotic agent.

Mon Apr 03 00:00:00 EDT 2023

Dietary Evodiamine Inhibits Atherosclerosis-Associated Changes in Vascular Smooth Muscle Cells.

International journal of molecular sciences

Zha Y,Yang Y,Zhou Y,Ye B,Li H,Liang J

Mon Apr 03 00:00:00 EDT 2023

Effect of mechanical tension on fibroblast transcriptome profile and regulatory mechanisms of myocardial collagen turnover.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Shiraishi M,Suzuki K,Yamaguchi A

436000 was used in Immunocytochemistry and Immunohistochemistry to show that Selenbp1 to be one of the genes responsible for regulating differentiation of fibroblasts in myocardial collagen.

Sat Apr 01 00:00:00 EDT 2023

Effect of mechanical tension on fibroblast transcriptome profile and regulatory mechanisms of myocardial collagen turnover.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Shiraishi M,Suzuki K,Yamaguchi A

436000 was used in Immunocytochemistry and Immunohistochemistry to show that Selenbp1 to be one of the genes responsible for regulating differentiation of fibroblasts in myocardial collagen.

Sat Apr 01 00:00:00 EDT 2023

EIF5A2 Is Involved in the Biological Process of Cervical Cancer Cells through AGR2.

Pharmacology

Shen X,Li L,He Y,Lv X,Ma J

436000 was used in Western Blotting to show that EIF5A2 knockdown inhibited the biological process of cervical cancer cells through modulation of AGR2.

Wed Jul 06 00:00:00 EDT 2022

Ciliary Neurotrophic Factor Modulates Multiple Downstream Signaling Pathways in Prostate Cancer Inhibiting Cell Invasiveness.

Cancers

Tossetta G,Fantone S,Gesuita R,Goteri G,Senzacqua M,Marcheggiani F,Tiano L,Marzioni D,Mazzucchelli R

436000 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that CNTF is expressed in PCa and CRPC tissues, and has a pivotal role in prostate cancer environment remodeling and as a negative modulator of invasion processes of CRPC cell models.

Wed Nov 30 00:00:00 EST 2022

Neuroprotective effects of violacein in a model of inherited amyotrophic lateral sclerosis.

Scientific reports

Olivera-Bravo S,Bolatto C,Otero Damianovich G,Stancov M,Cerri S,Rodríguez P,Boragno D,Hernández Mir K,Cuitiño MN,Larrambembere F,Isasi E,Alem D,Canclini L,Marco M,Davyt D,Díaz-Amarilla P

436000 was used in Immunohistochemistry-immunofluorescence to study if the pigment violacein with several reported protective/antiproliferative properties may control highly neurotoxic astrocytes (AbAs) obtained from spinal cord cultures of symptomatic hSOD1G93A rats.

Tue Mar 15 00:00:00 EDT 2022

Adiponectin and Leptin Exert Antagonizing Effects on HUVEC Tube Formation and Migration Modulating the Expression of CXCL1, VEGF, MMP-2 and MMP-9.

International journal of molecular sciences

Nigro E,Mallardo M,Polito R,Scialò F,Bianco A,Daniele A

436000 was used in Western Blotting to investigate the effects following the treatment of primary human umbilical vein endothelial cells with AdipoRon and/or leptin on proliferation, angiogenesis, and wound healing.

Tue Jul 13 00:00:00 EDT 2021

Adiponectin and Leptin Exert Antagonizing Effects on HUVEC Tube Formation and Migration Modulating the Expression of CXCL1, VEGF, MMP-2 and MMP-9.

International journal of molecular sciences

Nigro E,Mallardo M,Polito R,Scialò F,Bianco A,Daniele A

Tue Jul 13 00:00:00 EDT 2021

A hypoxia-induced Rab pathway regulates embryo implantation by controlled trafficking of secretory granules.

Proceedings of the National Academy of Sciences of the United States of America

Bhurke A,Kannan A,Neff A,Ma Q,Laws MJ,Taylor RN,Bagchi MK,Bagchi IC

436000 was used in Immunohistochemistry to provide an insight into the intracellular communication mechanisms that operate during adaptation to hypoxia, which is essential for embryo implantation and establishment of pregnancy.

Tue Jun 23 00:00:00 EDT 2020

Expression of metalloproteinases MMP-2 and MMP-9 is associated to the presence of androgen receptor in epithelial ovarian tumors.

Journal of ovarian research

Morales-Vásquez F,Castillo-Sánchez R,Gómora MJ,Almaraz MÁ,Pedernera E,Pérez-Montiel D,Rendón E,López-Basave HN,Román-Basaure E,Cuevas-Covarrubias S,Maldonado-Cubas J,Villa A,Mendez C

436000 was used in Immunohistochemistry to show that MMP-2 located in the epithelium and the stroma are independent prognostic biomarkers for overall survival in epithelial ovarian tumors.

Tue Jul 28 00:00:00 EDT 2020

Expression of metalloproteinases MMP-2 and MMP-9 is associated to the presence of androgen receptor in epithelial ovarian tumors.

Journal of ovarian research

Morales-Vásquez F,Castillo-Sánchez R,Gómora MJ,Almaraz MÁ,Pedernera E,Pérez-Montiel D,Rendón E,López-Basave HN,Román-Basaure E,Cuevas-Covarrubias S,Maldonado-Cubas J,Villa A,Mendez C

436000 was used in Immunohistochemistry to show that MMP-2 located in the epithelium and the stroma are independent prognostic biomarkers for overall survival in epithelial ovarian tumors.

Tue Jul 28 00:00:00 EDT 2020

MMP-2 and MMP-9 as prognostic markers for the early detection of urinary bladder cancer.

Journal of biochemical and molecular toxicology

Fouad H,Salem H,Ellakwa DE,Abdel-Hamid M

Published figure using MMP2 monoclonal antibody (Product # 436000) in Western Blot

Mon Apr 01 00:00:00 EDT 2019

MMP-2 and MMP-9 as prognostic markers for the early detection of urinary bladder cancer.

Journal of biochemical and molecular toxicology

Fouad H,Salem H,Ellakwa DE,Abdel-Hamid M

Published figure using MMP2 monoclonal antibody (Product # 436000) in Western Blot

Mon Apr 01 00:00:00 EDT 2019

Amphiregulin Regulates Phagocytosis-Induced Cell Death in Monocytes via EGFR and Matrix Metalloproteinases.

Mediators of inflammation

Platen C,Dreschers S,Reiss LK,Wappler J,Orlikowsky TW

436000 was used in Flow cytometry/Cell sorting to demonstrate that loss of AREG rescues PICD in CBMO to the level comparable to adult monocytes.

Tue Feb 19 00:00:00 EST 2019

The Activity of Matrix Metalloproteinases (MMP-2, MMP-9) and Their Tissue Inhibitors (TIMP-1, TIMP-3) in the Cerebral Cortex and Hippocampus in Experimental Acanthamoebiasis.

International journal of molecular sciences

Łanocha-Arendarczyk N,Baranowska-Bosiacka I,Gutowska I,Kolasa-Wołosiuk A,Kot K,Łanocha A,Metryka E,Wiszniewska B,Chlubek D,Kosik-Bogacka D

436000 was used in Immunohistochemistry to determine whether Acanthamoeba species may affect the levels of matrix metalloproteinases(MMPs)-2/-9, their tissue inhibitors, and MMP-9/TIMP-1, MMP-2/TIMP-3 ratios in the cerebral cortex and hippocampus, in relation to the host's immunological status.

Wed Dec 19 00:00:00 EST 2018

Lactobacillus casei Strain Shirota Enhances the In Vitro Antiproliferative Effect of Geniposide in Human Oral Squamous Carcinoma HSC-3 Cells.

Molecules (Basel, Switzerland)

Qian Y,Song JL,Sun P,Yi R,Liu H,Feng X,Park KY,Zhao X

436000 was used in Western Blotting to investigate the enhanced anti-proliferative effect of Lactobacillus casei strain Shirota on geniposide actions in human oral squamous carcinoma HSC-3 cells.

Thu May 03 00:00:00 EDT 2018

PARP-1 depletion in combination with carbon ion exposure significantly reduces MMPs activity and overall increases TIMPs expression in cultured HeLa cells.

Radiation oncology (London, England)

Ghorai A,Sarma A,Chowdhury P,Ghosh U

Published figure using MMP2 monoclonal antibody (Product # 436000) in Western Blot

Thu Sep 22 00:00:00 EDT 2016

Diurnal variation of tight junction integrity associates inversely with matrix metalloproteinase expression in Xenopus laevis corneal epithelium: implications for circadian regulation of homeostatic surface cell desquamation.

PloS one

Wiechmann AF,Ceresa BP,Howard EW

Published figure using MMP2 monoclonal antibody (Product # 436000) in Immunofluorescence

Tue Dec 22 00:00:00 EST 2015

PloS one

Wiechmann AF,Ceresa BP,Howard EW

Published figure using MMP2 monoclonal antibody (Product # 436000) in Immunofluorescence

Tue Dec 22 00:00:00 EST 2015

Lung dysfunction causes systemic hypoxia in estrogen receptor beta knockout (ERbeta-/-) mice.

Proceedings of the National Academy of Sciences of the United States of America

Morani A,Barros RP,Imamov O,Hultenby K,Arner A,Warner M,Gustafsson JA

436000 was used in western blot to study the cause of systemic hypoxia in estrogen receptor beta knockout (Erbeta-/-) mice by lung dysfunction

Tue May 02 00:00:00 EDT 2006

生物信息学

蛋白别名: 72 kDa gelatinase; 72 kDa type IV collagenase; 72 kDa type IV collagenase isoform a preproprotein; 72kD gelatinase; 72kD type IV collagenase; 72kDa gelatinase; 72kDa type IV collagenase; CLG 4A; Collagenase; collagenase type IV-A; Gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase 2; Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP-2; MMPs; neutrophil gelatinase; Progelatinase A; TBE-1; TBE1

查看更多收起

基因别名: CLG4; CLG4A; GelA; MMP-2; MMP-II; MMP2; MONA; TBE-1

查看更多收起

功能

metalloendopeptidase activity serine-type endopeptidase activity protein binding metallopeptidase activity zinc ion binding fibronectin binding peptidase activity hydrolase activity metal ion binding metalloprotease

参与通路

angiogenesis response to hypoxia blood vessel maturation intramembranous ossification proteolysis embryo implantation extracellular matrix disassembly collagen catabolic process endodermal cell differentiation cellular protein metabolic process positive regulation of innate immune response ephrin receptor signaling pathway face morphogenesis bone trabecula formation cellular response to amino acid stimulus positive regulation of vascular smooth muscle cell proliferation ovarian follicle development ovulation from ovarian follicle luteinization response to oxidative stress negative regulation of cell adhesion female pregnancy parturition aging response to radiation response to mechanical stimulus peripheral nervous system axon regeneration response to activity cell migration positive regulation of cell migration response to retinoic acid response to cytokine response to nicotine response to drug response to hydrogen peroxide positive regulation of apoptotic process response to estrogen negative regulation of vasoconstriction skeletal system morphogenesis tissue remodeling response to electrical stimulus response to hyperoxia prostate gland epithelium morphogenesis cellular response to cytokine stimulus cellular response to interleukin-1 cellular response to estradiol stimulus cellular response to fluid shear stress

MMP2 Monoclonal Antibody (101)

MMP2 Antibody (436000) in ICC/IF

产品信息

436000

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

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