Invitrogen

MMP9 Monoclonal Antibody (L51/82), PerCP

MMP9 Antibody in Immunocytochemistry (ICC/IF)

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MMP9 Antibody (MA5-45513) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using NIH 3T3 (NIH 3T3). Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with MMP9 monoclonal antibody (Product # MA5-45513) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C)... View More

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产品信息

MA5-45513

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

L51/82

抗原

Fusion protein amino acids 1-708 (full length) of rat MMP9

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偶联物

PerCP PerCP PerCP

激发/发射光谱

482/675 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2931967

产品详细信息

1 µg/mL of MA5-45513 was sufficient for detection of MMP9 in 20 µg of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 92kDa and approximately 82kDa (pro and active forms).

This antibody was formerly sold as S51-82.

靶标信息

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 92 kDa gelatinase; 92 kDa type IV collagenase; 92-kDa type IV collagenase; 92kD gelatinase; 92kD type IV collagenase; 92kDa gelatinase; 92kDa type IV collagenase; collagenase type IVB; Gel B; Gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; matrix metallo protease; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix metalloproteinase-9; Matrix metalloproteinase9; MMP; MMP-9; MMPs; type V collagenase

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基因别名: AW743869; B/MMP9; CLG4B; GELB; MANDP2; MMP-9; MMP9; pro-MMP-9

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UniProt ID: (Human) P14780, (Rat) P50282, (Mouse) P41245

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Entrez Gene ID: (Human) 4318, (Rat) 81687, (Mouse) 17395

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