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MSH3 Antibody (PA5-114764) in ICC/IF
Immunocytochemistry analysis of MSH3 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114764). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
产品信息
PA5-114764
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MSH3(Accession P20585), corresponding to amino acid residues G110-F160.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2899400
产品详细信息
Antibody detects endogenous levels of total MSH3.
靶标信息
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Divergent upstream protein; DNA mismatch repair protein Msh3; DUP; hMSH3; Mismatch repair protein 1; MRP1; Protein repair-1; Protein repair-3; REP-1; REP-3
基因别名: D13Em1; DUC1; DUG; DUP; MRP1; MSH3; Rep-3; Rep3
UniProt ID: (Human) P20585, (Mouse) P13705
Entrez Gene ID: (Human) 4437, (Rat) 499505, (Mouse) 17686
damaged DNA binding
single-stranded DNA binding
protein binding
ATP binding
enzyme binding
mismatched DNA binding
guanine/thymine mispair binding
dinucleotide insertion or deletion binding
single guanine insertion binding
dinucleotide repeat insertion binding
oxidized purine DNA binding
protein homodimerization activity
Y-form DNA binding
heteroduplex DNA loop binding
double-strand/single-strand DNA junction binding
molecular_function
DNA binding
DNA-dependent ATPase activity
centromeric DNA binding
nucleotide binding
DNA metabolism protein
meiotic mismatch repair
DNA repair
mismatch repair
reciprocal meiotic recombination
maintenance of DNA repeat elements
negative regulation of DNA recombination
positive regulation of helicase activity
biological_process
somatic hypermutation of immunoglobulin genes
somatic recombination of immunoglobulin gene segments
cellular response to DNA damage stimulus
