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MTHFD2 Antibody (PA5-106331) in ICC/IF
Immunofluorescent analysis of MTHFD2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and MTHFD2 polyclonal antibody (Product # PA5-106331) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-106331
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MTHFD2(Accession P13995), corresponding to amino acid residues G42-A92.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2854008
产品详细信息
Antibody detects endogenous levels of total MTHFD2.
靶标信息
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase; methylenetetrahydrofolate dehydrogenase 2; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase
基因别名: AW558851; MTHFD2; NMDMC
UniProt ID: (Human) P13995, (Mouse) P18155
Entrez Gene ID: (Human) 10797, (Mouse) 17768, (Rat) 680308
magnesium ion binding
formate-tetrahydrofolate ligase activity
methenyltetrahydrofolate cyclohydrolase activity
methylenetetrahydrofolate dehydrogenase (NAD+) activity
methylenetetrahydrofolate dehydrogenase (NADP+) activity
phosphate ion binding
catalytic activity
oxidoreductase activity
hydrolase activity
molecular_function
