Invitrogen

MTHFD2 Polyclonal Antibody

MTHFD2 Antibody in Immunocytochemistry (ICC/IF)

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MTHFD2 Antibody (PA5-28169) in ICC/IF

MTHFD2 Polyclonal Antibody detects MTHFD2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTHFD2 stained by MTHFD2 Polyclonal Antibody (Product # PA5-28169) diluted at 1:500. Blue: Fluoroshield with DAPI .

MTHFD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MTHFD2 Antibody in Immunoprecipitation (IP)

MTHFD2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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产品信息

PA5-28169

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 30 and 304 of Human MTHFD2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.13 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2545645

产品详细信息

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, SK-N-DZ, SK-N-SH.

Predicted reactivity: Mouse (95%), Rat (93%), Zebrafish (86%), Xenopus laevis (90%), Chicken (92%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MTHFD2 facilitates breast cancer cell proliferation via the AKT signaling pathway.

Experimental and therapeutic medicine

Huang J,Qin Y,Lin C,Huang X,Zhang F

PA5-28169 was used in Western Blot to provide an alternative theoretical foundation that could help the development of MTHFD2-targeted breast cancer treatment.

Thu Jul 01 00:00:00 EDT 2021

Cardiac metabolic pathways affected in the mouse model of barth syndrome.

PloS one

Huang Y,Powers C,Madala SK,Greis KD,Haffey WD,Towbin JA,Purevjav E,Javadov S,Strauss AW,Khuchua Z

PA5-28169 was used in immunohistochemistry - frozen section and western blot to study the effect of Taz-knockdown on the mitochondrial processes in cardiac tissues

Wed Apr 06 00:00:00 EDT 2016

Cardiac metabolic pathways affected in the mouse model of barth syndrome.

PloS one

Huang Y,Powers C,Madala SK,Greis KD,Haffey WD,Towbin JA,Purevjav E,Javadov S,Strauss AW,Khuchua Z

PA5-28169 was used in immunohistochemistry - frozen section and western blot to study the effect of Taz-knockdown on the mitochondrial processes in cardiac tissues

Wed Apr 06 00:00:00 EDT 2016

生物信息学

蛋白别名: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase

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基因别名: MTHFD2; NMDMC

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UniProt ID: (Human) P13995

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Entrez Gene ID: (Human) 10797

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功能

magnesium ion binding formate-tetrahydrofolate ligase activity methenyltetrahydrofolate cyclohydrolase activity methylenetetrahydrofolate dehydrogenase (NAD+) activity methylenetetrahydrofolate dehydrogenase (NADP+) activity phosphate ion binding

参与通路

one-carbon metabolic process folic acid-containing compound biosynthetic process tetrahydrofolate metabolic process folic acid metabolic process oxidation-reduction process