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Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is small intestine tissue.
Secreted epithelial mucins are large macromolecules which exhibit extreme polydispersity. Mucin 2 is the major intestinal mucin. O-glycans are attached to MUC2 in a potentially diverse arrangement, which is crucial for their interaction with endogeneous and exogeneous lectins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Intestinal mucin-2; MUC-2; mucin 2, intestinal/tracheal; Mucin-2; mucin-like protein
基因别名: MLP; MUC-2; MUC2; SMUC
UniProt ID: (Human) Q02817
Entrez Gene ID: (Human) 4583
分子生物学功能:
extracellular matrix protein