Invitrogen

MUSK Polyclonal Antibody

MUSK Antibody in Immunocytochemistry (ICC/IF)

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MUSK Antibody (PA1-1741) in ICC/IF

Immunofluorescent analysis of muscle-specific kinase (MuSK) (green) in C2C12 mouse myoblast cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained without (left panel) or with a MuSK polyclonal antibody (Product # PA1-1741, right panel), at a dilution of 1:20 in blocking buffer for at least 1 hour at room temperature, and then incubated with a DyLight 488 goat anti-rabbit IgG secondary... View More

MUSK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA1-1741

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20-1:100

免疫细胞化学 (ICC/IF)

1:20-1:100

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免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein containing residues 210-304 from the extracellular domain of rat MuSK

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2147253

产品详细信息

PA1-1741 detects a predominant band at ~98kD. Other lower MW nonspecific bands of unknown identity were also detected and are likely to be proteolytic fragments.

靶标信息

MUSK plays an important and selective role in many aspects of skeletal muscle development and function through the initial step of postsynaptic differentiation and formation of end-plate bands in cooperation with Rapsyn. Communication between cells is often mediated by receptors on the surface of the target cell that are triggered by specific ligands released by other cells. Receptor tyrosine kinase activity is strictly regulated by the binding of the appropriate ligand to the extracellular active site of the receptor. MuSK has been shown to be part of the agrin signaling complex and plays an important role in the development and architectural maintenance of the neuromuscular junction.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction.

eLife

Afshar Bakooshli M,Lippmann ES,Mulcahy B,Iyer N,Nguyen CT,Tung K,Stewart BA,van den Dorpel H,Fuehrmann T,Shoichet M,Bigot A,Pegoraro E,Ahn H,Ginsberg H,Zhen M,Ashton RS,Gilbert PM

PA1-1741 was used in Immunocytochemistry-immunoflourescence to evaluate and model 3D human neuromuscular junction de novo development or disease in culture.

Tue May 14 00:00:00 EDT 2019

Interaction between ROR1 and MuSK activation complex in myogenic cells.

FEBS letters

Karvonen H,Summala K,Niininen W,Barker HR,Ungureanu D

PA1-1741 was used in Immunoprecipitation to identify MuSK as a ROR1 as a new interacting partner for MuSK and Dok-7, which may have an important role in myogenic cell signalling.

Thu Feb 01 00:00:00 EST 2018

Interaction between ROR1 and MuSK activation complex in myogenic cells.

FEBS letters

Karvonen H,Summala K,Niininen W,Barker HR,Ungureanu D

PA1-1741 was used in Immunoprecipitation to identify MuSK as a ROR1 as a new interacting partner for MuSK and Dok-7, which may have an important role in myogenic cell signalling.

Thu Feb 01 00:00:00 EST 2018

The Agrin/MuSK signaling pathway is spatially segregated from the neuregulin/ErbB receptor signaling pathway at the neuromuscular junction.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Trinidad JC,Fischbach GD,Cohen JB

PA1-1741 was used in immunohistochemistry to investigate the distribution of protein components of the neuregulin/erbB receptor and agrin/MuSK pathways at NMJs of adult rat gastrocnemius muscle

Fri Dec 01 00:00:00 EST 2000

Laminin and alpha-dystroglycan mediate acetylcholine receptor aggregation via a MuSK-independent pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Montanaro F,Gee SH,Jacobson C,Lindenbaum MH,Froehner SC,Carbonetto S

PA1-1741 was used in immunoprecipitation to investigate the regulation of AChR aggregation by laminin and alpha-dystroglycan

Sun Feb 15 00:00:00 EST 1998

Dimerization of the muscle-specific kinase induces tyrosine phosphorylation of acetylcholine receptors and their aggregation on the surface of myotubes.

The Journal of biological chemistry

Hopf C,Hoch W

PA1-1741 was used in western blot to investigate the effect of MuSK dimerization on acetylcholine receptor's tyrosine phosphorylation and aggregation

Fri Mar 13 00:00:00 EST 1998

生物信息学

蛋白别名: MGC126323; MGC126324; muscle localized kinase 2; muscle specific kinase (neural fold/somite kinase 1); Muscle, skeletal receptor tyrosine protein kinase; Muscle, skeletal receptor tyrosine-protein kinase; muscle, skeletal, receptor tyrosine kinase; muscle-specific kinase receptor; muscle-specific protein kinase secretory isoform; muscle-specific tyrosine kinase receptor MuSK; Muscle-specific tyrosine protein kinase receptor; Muscle-specific tyrosine-protein kinase receptor; MuSK; RP23-170A3.1

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基因别名: CMS9; FADS; Mdk4; Mlk; MUSK; Nsk1; Nsk2; Nsk3

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UniProt ID: (Human) O15146, (Mouse) Q61006, (Rat) Q62838

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Entrez Gene ID: (Human) 4593, (Mouse) 18198, (Rat) 81725

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