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MYH9 Antibody (MA5-27765) in IHC (P)
MYH9 Monoclonal Antibody (GT218) detects MYH9 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MYH9 Monoclonal Antibody (GT218) (Product # MA5-27765) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
产品信息
MA5-27765
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Dog
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
GT218
抗原
Recombinant protein encompassing a sequence within the N-terminus region of human MYH9. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7, with 20% glycerol
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2735282
产品详细信息
Positive Control: A431
Predicted Reactivity: Rat (97%), Xenopus laevis (89%), Dog (99%), Chicken (94%), Bovine (99%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The MYH9 gene, located on chromosome 22q12.3, encodes the heavy chain of non-muscle myosin IIA (NMHC IIA), a critical component of the actin cytoskeleton that plays essential roles in various cellular processes. Structurally, the MYH9 gene spans over 106 kilobases and includes 41 exons that translate into a protein of 1,960 amino acids. This protein is a part of a hexameric complex, which includes two heavy chains, two regulatory light chains, and two essential light chains. The NMHC IIA protein interacts with actin filaments and is involved in cellular activities such as cell migration, adhesion, division, and maintenance of cell shape. Functionally, mutations in MYH9 can result in a spectrum of autosomal dominant disorders collectively known as MYH9-related diseases (MYH9-RD), which include conditions such as May-Hegglin anomaly, Fechtner syndrome, and Epstein syndrome. These disorders are primarily characterized by macrothrombocytopenia (abnormally large platelets) and may lead to other complications such as hearing loss, renal failure, and cataracts later in life. MYH9 is also crucial in hematopoiesis, where its proper function is necessary for the survival and maintenance of hematopoietic stem and progenitor cells (HSPCs). Loss of MYH9 function disrupts normal hematopoiesis, leading to severe blood cell deficiencies and bone marrow failure.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cellular myosin heavy chain, type A; flectin; MGC104539; Myosin heavy chain 9; myosin heavy chain IX; Myosin heavy chain, non-muscle IIa; myosin IIA; Myosin-9; myosin9; NMHC II-A; NMHC-II A; NMMHC II-a; NMMHC IIA; NMMHC-A; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A
基因别名: BDPLT6; DFNA17; EPSTS; Fltn; FTNS; MHA; MYH9; Myhn-1; Myhn1; NMHC-II-A; NMHCIIA; NMMHC-A; NMMHC-IIA; NMMHCA; TU72.6
UniProt ID: (Human) P35579, (Mouse) Q8VDD5
Entrez Gene ID: (Human) 4627, (Mouse) 17886
microfilament motor activity
motor activity
actin binding
protein binding
calmodulin binding
ATP binding
ATPase activity
protein domain specific binding
actin-dependent ATPase activity
protein homodimerization activity
protein anchor
ADP binding
poly(A) RNA binding
actin filament binding
cadherin binding involved in cell-cell adhesion
nucleotide binding
meiotic spindle organization
cell morphogenesis involved in differentiation
cytokinesis
angiogenesis
in utero embryonic development
establishment of T cell polarity
membrane protein ectodomain proteolysis
phagocytosis, engulfment
movement of cell or subcellular component
cell adhesion
myoblast fusion
regulation of cell shape
protein transport
single organismal cell-cell adhesion
actin filament polymerization
actin filament-based movement
platelet formation
actomyosin structure organization
actin cytoskeleton reorganization
uropod organization
blood vessel endothelial cell migration
establishment of meiotic spindle localization
actin filament capping
platelet aggregation
negative regulation of actin filament severing
positive regulation of protein processing in phagocytic vesicle
integrin-mediated signaling pathway
monocyte differentiation
leukocyte migration
