Invitrogen

MYOCD Monoclonal Antibody (355521)

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MYOCD Antibody in Western Blot (WB)

MYOCD Antibody in Western Blot (WB)

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MYOCD Antibody (MA5-24103) in WB

Western blot analysis of MYOCD in MCF‚7 human breast cancer cell line, Raji human Burkitts lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. Samples were incubated in MYOCD monoclonal antibody (Product # MA5-24103) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions.

MYOCD Antibody in Western Blot (WB)

MYOCD Antibody in Western Blot (WB)

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MYOCD Antibody in Western Blot (WB) — Western blot analysis of MYOCD in MCF‚7 human breast cancer cell line, Raji human Burkitts lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. Samples were incubated in MYOCD monoclonal antibody (Product # MA5-24103) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions.

MYOCD Antibody in Western Blot (WB) — Figure 5 CircYTHDC2 negatively regulates TET2. (A) The schematic diagram of TET2-WT or TET2-Mut luciferase reporter gene. The wild type motifs were indicated by green and the mutant motif was indicated by red. A7R5 cells were transfected with TET2-WT or TET2-Mut, combined with circYTHDC2 siRNA. The cells transfected with scramble control were used as control. Luciferase report gene assay were performed to measure the relative luciferase activity. (B) Relative enrichment representing TET2 and circYTHDC2 RNA levels associated with circYTHDC2 junction compared to control. (C) qRT-PCR analysis for the expression of TET2 after high glucose treatment in A7R5 cells. (D) western blotting analysis of TET2 expression after high glucose treatment in A7R5 cells. (E) qRT-PCR analysis for the expression of TET2 after circYTHDC2 siRNA transfection in A7R5 cells. (F) western blotting analysis of TET2 expression after circYTHDC2 siRNA transfection in A7R5 cells. (G) western blotting analysis of TET2 expression after TET2 siRNA transfection in A7R5 cells. (H) western blotting analysis for the expression of MYODC, SRF and KLF4 in A7R5 cells after circYTHDC2 siRNA transfection alone, or combined with TET2 siRNA transfection. (I) qRT-PCR analysis for the expression of TET2 after circYTHDC2 transfection in A7R5 cells. (J) western blotting analysis of TET2 expression after circYTHDC2 transfection in A7R5 cells. (K) western blotting analysis of TET2 expression after TET2 transfection in A7R5 cells.

产品信息

MA5-24103

应用

建议稀释比

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免疫印迹 (WB)

1 µg/mL

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产品规格

种属反应

Human, Mouse

已发表种属

Rat

宿主/亚型

Mouse / IgG2B

分类

Monoclonal

类型

Antibody

克隆号

355521

抗原

E. coli-derived recombinant human Myocardin Met97-Leu290

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2606771

产品详细信息

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

YTHDC2-Mediated circYTHDC2 N6-Methyladenosine Modification Promotes Vascular Smooth Muscle Cells Dysfunction Through Inhibiting Ten-Eleven Translocation 2.

Frontiers in cardiovascular medicine

Yuan J,Liu Y,Zhou L,Xue Y,Lu Z,Gan J

Published figure using MYOCD monoclonal antibody (Product # MA5-24103) in Western Blot

Fri Oct 22 00:00:00 EDT 2021

YTHDC2-Mediated circYTHDC2 N6-Methyladenosine Modification Promotes Vascular Smooth Muscle Cells Dysfunction Through Inhibiting Ten-Eleven Translocation 2.

Frontiers in cardiovascular medicine

Yuan J,Liu Y,Zhou L,Xue Y,Lu Z,Gan J

Published figure using MYOCD monoclonal antibody (Product # MA5-24103) in Western Blot

Fri Oct 22 00:00:00 EDT 2021

生物信息学

蛋白别名: Basic SAP coiled-coil transcription activator 2; Myocardin; SRF co-factor protein (cardiac and smooth muscle); SRF cofactor protein

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基因别名: Bsac2; BSAC2A; MYCD; MYOCD; Srfcp

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UniProt ID: (Human) Q8IZQ8, (Mouse) Q8VIM5

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Entrez Gene ID: (Human) 93649, (Mouse) 214384

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core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein binding transcription factor binding histone acetyltransferase binding histone deacetylase binding R-SMAD binding transcription coactivator activity DNA-binding transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter regulation of cell growth by extracellular stimulus vasculogenesis response to hypoxia cardiac ventricle development positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription from RNA polymerase II promoter positive regulation of cell proliferation negative regulation of cell proliferation negative regulation of cardiac muscle cell apoptotic process negative regulation of myotube differentiation positive regulation of transforming growth factor beta receptor signaling pathway cardiocyte differentiation regulation of histone acetylation hepatic stellate cell activation vascular smooth muscle cell differentiation positive regulation of DNA binding cellular component maintenance regulation of myoblast differentiation negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle contraction lung alveolus development digestive tract development positive regulation of sequence-specific DNA binding transcription factor activity smooth muscle cell differentiation regulation of smooth muscle cell differentiation positive regulation of smooth muscle cell differentiation cardiac muscle cell differentiation ventricular cardiac muscle cell differentiation uterus development urinary bladder development cell growth involved in cardiac muscle cell development cellular response to growth factor stimulus cellular response to hypoxia ductus arteriosus closure negative regulation of beta-amyloid clearance regulation of phenotypic switching positive regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation positive regulation of cardiac vascular smooth muscle cell differentiation positive regulation of cardiac muscle cell differentiation negative regulation of skeletal muscle cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter heart development muscle cell differentiation