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Mesothelioma Antibody (MA2-310) in ICC/IF
Immunofluorescent analysis of Mesothelioma in MCF-7 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Mesothelioma monoclonal antibody (Product # MA2-310) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Mesothelioma staining (green), F... View More
产品信息
MA2-310
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
ME1
抗原
Mesothelioma cell line SPC111.
偶联物
形式
Lyophilized
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_325451
产品详细信息
MA2-310 detects a surface membrane glycoprotein preferentially expressed on normal and malignant mesothelial cells from human tissues. This antibody does not detect most lung adenocarcinoma cell lines, or well and moderately differentiated pulmonary adenocarcinomas.
MA2-310 has been successfully used in indirect immunofluorescence, immunocytochemistry, immunohistochemistry and solid-phase radioimmunoassay procedures. This antibody does not react in Western blot procedures. Immunohistochemical staining of sarcomatoid mesotheliomas with MA2-310 results in intense staining of mesothelial cells.
It has been reported that this antibody reacts with a 200 kDa surface membrane glycoprotein preferentially expressed on normal and malignant mesothelial cells, but does not react with most lung adenocarcinoma cell lines, or well and moderately differentiated pulmonary adenocarcinomas. In studies, this antibody was shown to react with all mesotheliomas examined, with 50%-99% of cells of both pleural and peritoneal mesotheliomas stained. Literature reports have indicated that the antibody does not bind with many common breast (MCF-7, T47D, ZR75-1, BT20, SJC38), ovarian (OVCA429, OVCA432, OVCA434), colon (SW480, SW620, CX1, WIDR, Co112), and renal-cell (FOHN, ZNH1) carcinoma cell lines, nor with several leukemia (K562, U937, CEM) or fibroblast (WI38, R2F) cell lines. Some reactivity has been reported with some pulmonary tumors of squamous-cell and small-cell carcinoma origin, adenocarcinoma of the breast, and ovary, and gastrointestinal adenocarcinomas. Also, melanomas and poorly differentiated pulmonary adenocarcinoma tumors have reacted with this antibody.
MA2-310 will not react with formalin-fixed, or alcohol-fixed, paraffin embedded tissues. Use of frozen, acetone fixed sections or cell suspensions is recommended.
The MA2-310 antigen is mesothelioma cell line SPC111.
Reconstitute with PBS.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
