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Invitrogen
Mouse anti-Human IgG Fc (CH2 domain) Secondary Antibody, HRP
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Human IgG Fc (CH2 domain) Secondary Antibody (MA5-16859) in WB
Western blot (non-reducing) was performed Mouse anti-Human IgG Fc (CH2 domain) Secondary Antibody, HRP (Product # MA5-16859) and a 150 kDa band corresponding toHuman IgG was observed in IM-9, ARH-77 and IM-9, ARH-77 conditioned medium8203(CM) but not in Raji,Jurkat, Molt-4 andJurkatCM which are known to have lowexpression. Whole cell lysate (30 µg) of IM-9 (Lane 1), ARH-77 (Lane 2), Raji (Lane 3), Jurkat (L... View More
产品信息
MA5-16859
应用
建议稀释比
免疫印迹 (WB)
1:2,000
免疫组化（冰冻） (IHC (F))
1:100 -1:200
酶联免疫吸附实验 (ELISA)
1:1,000-1:10,000
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Secondary Antibody
克隆号
MK 1 A6
抗原
Human IgG Polyclonal
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
affinity chromatography
保存液
PBS
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2538338
靶标
IgG Fc
抗体形式
Whole Antibody
产品详细信息
This antibody recognizes human IgG Fc (all subclasses). The epitope detected lies within the CH2 domain as determined by haemagglutination and western blotting using IgG heavy chain and myelomas with defined domain deletions. The epitope is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. MA5-16859 cross-reacts with Rhesus monkey.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
