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Invitrogen
Mouse anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Human IgG (H+L) Cross-Adsorbed Secondary Antibody (31137) in ICC/IF
Immunofluorescence analysis of Mouse anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody was performedusing log phase IM9 cells (IgG producing B-cell line). The cells were fixed with 4% paraformaldehyde for 10 minutes,permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cellswere labeled with Mouse anti-Human IgG (H+L) Cross-Adsorbed Secondary A... View More
产品信息
31137
应用
建议稀释比
免疫印迹 (WB)
1 µg/mL
免疫组化 (IHC)
Assay-dependent
免疫细胞化学 (ICC/IF)
Assay-dependent
流式细胞分析 (Flow)
Assay-dependent
免疫沉淀 (IP)
Assay-dependent
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Liquid
浓度
1.7 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6
内含物
no preservative
保存条件
4° C
运输条件
Ambient
RRID
AB_228371
靶标
IgG
交叉吸附
Against bovine, horse and mouse serum proteins
抗体形式
Whole Antibody
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31137 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31137 reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, horse and mouse serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions.
Country of Origin: USA
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
