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Human IgM Secondary Antibody (SA1-72512) in WB
Western blot analysis of Human IgM in various protein samples. Samples were incubated with Mouse anti-Human IgM Secondary Antibody (Product # SA1-72512) with a dilution of 1 µg/mL, followed by peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgM band was visualized using ECL Western Blotting Substrate. Lane 1: 100 ng of human IgG Lane 2: 100 ng of human IgA Lane 3: 100 ng of human IgM Lane 4: 100 ng of human IgE Result: KT16 can specifically detect human IgM by Western blotting.
产品信息
SA1-72512
应用
建议稀释比
免疫印迹 (WB)
1:1,000
酶联免疫吸附实验 (ELISA)
1:200
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Secondary Antibody
克隆号
KT16
抗原
Recombinant human IgM
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_923380
靶标
IgM
抗体形式
Whole Antibody
产品详细信息
This antibody reacts with the IgM chain C region and does not cross-react with human IgG and recombinant human IgG1, IgG2, IgG3, IgG4, IgA or IgE. This can be used in an Indirect ELISA (1 µg/mL) or as a capture antibody in a sandwich ELISA (5 µg/mL).
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
