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Rat IgG1 Secondary Antibody (12-4812-82) in Flow
Staining of mouse splenocytes with Rat IgG1 K Isotype Control Purified (Product # 14-4301-82) (left) or Anti-Mouse CD25 Purified (Product # 14-0251-86) (right), followed by staining with Anti-Mouse CD4 PerCP-eFluor® 710 (Product # 46-0042-82) and 0.125 µg of Anti-Rat IgG1 PE. Cells in the lymphocyte gate were used for analysis.
产品信息
12-4812-82
应用
建议稀释比
流式细胞分析 (Flow)
0.25 µg/test
产品规格
种属反应
Rat
宿主/亚型
Mouse
/ IgG
分类
Monoclonal
类型
Secondary Antibody
克隆号
R1-12D10
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572613
靶标
IgG1
抗体形式
Whole Antibody
产品详细信息
Description: The R1-12D10 monoclonal antibody recognizes rat IgG1 antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG1.
Applications Reported: This R1-12D10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This R1-12D10 antibody has been tested by flow cytometric analysis of mouse splenocytes stained with a rat IgG1 primary antibody. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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