Invitrogen

NCF2 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1个已发表图片

2 篇参考文献

查看其他12个NCF2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

NCF2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

NCF2 Antibody (PA5-37323) in ICC/IF

Immunofluorescence analysis of NCF2 in HeLa cells. Samples were incubated with NCF2 polyclonal antibody (Product # PA5-37323).

NCF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

NCF2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of NCF2 was observed in all cell lines tested in comparison to MCF7 using product (Product # PA5-37323) in western blot. {RE}

产品信息

PA5-37323

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant full length human NCF2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2554008

产品详细信息

This antibody detects endogenous protein at a molecular weight of 60 kDa.

Purity is >95% by SDS-PAGE.

靶标信息

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Defining blood-induced microglia functions in neurodegeneration through multiomic profiling.

Nature immunology

Mendiola AS,Yan Z,Dixit K,Johnson JR,Bouhaddou M,Meyer-Franke A,Shin MG,Yong Y,Agrawal A,MacDonald E,Muthukumar G,Pearce C,Arun N,Cabriga B,Meza-Acevedo R,Alzamora MDPS,Zamvil SS,Pico AR,Ryu JK,Krogan NJ,Akassoglou K

PA5-37323 was used in Western Blotting to provide an interactive resource for investigation of the immunology of blood proteins that could support therapeutic targeting of microglia activation by immune and vascular signals.

Sat Jul 01 00:00:00 EDT 2023

Natural variation in macrophage polarization and function impact pneumocyte senescence and susceptibility to fibrosis.

Aging

Chung EJ,Kwon S,Shankavaram U,White AO,Das S,Citrin DE

Published figure using NCF2 polyclonal antibody (Product # PA5-37323) in Western Blot

Wed Sep 28 00:00:00 EDT 2022

Natural variation in macrophage polarization and function impact pneumocyte senescence and susceptibility to fibrosis.

Aging

Chung EJ,Kwon S,Shankavaram U,White AO,Das S,Citrin DE

Published figure using NCF2 polyclonal antibody (Product # PA5-37323) in Western Blot

Wed Sep 28 00:00:00 EDT 2022

生物信息学

蛋白别名: 67 kDa neutrophil oxidase factor; chronic granulomatous disease, autosomal 2; FLJ93058; NADPH oxidase activator 2; NADPH oxidase subunit (67 kD); NADPH oxidase subunit (67kDa); NCF-2; ncf2; ncf2 {ECO:0000312|EMBL:FAA00361.1}; Neutrophil cytosol factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2); Neutrophil NADPH oxidase factor 2; p67-phox

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基因别名: NCF-2; NCF2; NOXA2; P67-PHOX; P67PHOX

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UniProt ID: (Human) P19878, (Mouse) O70145

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Entrez Gene ID: (Human) 4688, (Mouse) 17970, (Rat) 364018

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功能

protein binding protein C-terminus binding electron carrier activity superoxide-generating NADPH oxidase activity Rac GTPase binding molecular_function protein-binding activity modulator

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