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NEK2 Antibody (MA1-34594) in WB
Western blot was performed using Anti-NEK2 Monoclonal Antibody (Product # MA1-34594) and a 52 kDa band corresponding to NEK2 was observed across all the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HEK-293 (Lane 1), MCF7 (Lane 2) and Hep G2 (Lane 3) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary a... View More
产品信息
MA1-34594
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
1B6
抗原
Synthetic peptide Ð Immunogen sequence information is commercially sensitive
偶联物
形式
Liquid
浓度
0.43 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1958903
产品详细信息
Recommended positive controls: Jurkat cell lysate.
靶标信息
NEK2 is involved in cell cycle regulation and is essential for faithful chromosome segregation. NEK2 is a gene that encodes for a serine/threonine-protein kinase. This protein plays a crucial role in the regulation of mitosis and is primarily located in the centrosome. During the G1 phase, the protein is undetectable, but its levels progressively increase throughout the S phase, reaching their peak in late G2 phase. The gene has several alternatively spliced transcript variants that encode different isoforms with distinct C-termini. NEK2 is associated with several diseases, including Retinitis Pigmentosa 67 and Retinitis Pigmentosa.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HSPK 21; NEK 2; NEK 2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NIMA-related kinase 2; NimA-related protein kinase 2; NLK 1; protein phosphatase 1, regulatory subunit 111; Serine/threonine-protein kinase Nek2
基因别名: HsPK21; NEK2; NEK2A; NLK1; PPP1R111; RP67
UniProt ID: (Human) P51955
Entrez Gene ID: (Human) 4751
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
blastocyst development
protein phosphorylation
chromosome segregation
mitotic nuclear division
regulation of mitotic nuclear division
positive regulation of telomere maintenance via telomerase
negative regulation of DNA binding
regulation of mitotic centrosome separation
protein autophosphorylation
spindle assembly
centrosome separation
cell division
meiotic cell cycle
positive regulation of telomerase activity
regulation of attachment of spindle microtubules to kinetochore
mitotic spindle assembly
negative regulation of centriole-centriole cohesion
positive regulation of telomere capping
