Invitrogen

NF-H Polyclonal Antibody

NF-H Antibody in Immunocytochemistry (ICC/IF)

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NF-H Antibody (PA3-16753) in ICC/IF

Immunocytochemistry analysis of NF-H in mixed rat neuron/glial cultures. Samples were incubated in NF-H polyclonal antibody (Product # PA3-16753). Neurofilament Light (NF-L) antibody [green] and Neurofilament Heavy (NF-H) antibody. Blue is a DNA stain. NF-H antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The surrounding axonal profiles are orange due to staining of both the NF-H and NF-L antibodies.

NF-H Antibody in Immunohistochemistry - Free Floating (IHC (Free))

NF-H Antibody in Immunohistochemistry (Frozen) (IHC (F))

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产品信息

PA3-16753

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100,000

免疫组化（石蜡） (IHC (P))

1:5,000

免疫组化（冰冻） (IHC (F))

1:5,000

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免疫组织化学（悬浮细胞） (IHC (Free))

Assay-Dependent

免疫细胞化学 (ICC/IF)

1:20,000

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酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Bovine, Dog, Horse, Cat, Human, Mouse, Pig, Rat

已发表种属

Mouse

宿主/亚型

Chicken / IgY

分类

Polyclonal

类型

Antibody

抗原

Purified by NF-H protein from bovine intermediate filaments prepared from spinal cords.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

纯化类型

IgY fraction

保存液

tissue culture supernatant

内含物

0.035% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2149632

靶标信息

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SLC13A5/sodium-citrate co-transporter overexpression causes disrupted white matter integrity and an autistic-like phenotype.

Brain communications

Rigby MJ,Orefice NS,Lawton AJ,Ma M,Shapiro SL,Yi SY,Dieterich IA,Frelka A,Miles HN,Pearce RA,Yu JPJ,Li L,Denu JM,Puglielli L

PA3-16753 was used in Immunocytochemistry to test the hypothesis that modulating the intracellular flux of citrate through overexpression of SLC13A5, the plasma membrane sodium/citrate symporter, would result in an autistic-like phenotype in a mouse model.

Tue Feb 01 00:00:00 EST 2022

Increased expression of SLC25A1/CIC causes an autistic-like phenotype with altered neuron morphology.

Brain : a journal of neurology

Rigby MJ,Orefice NS,Lawton AJ,Ma M,Shapiro SL,Yi SY,Dieterich IA,Frelka A,Miles HN,Pearce RA,Yu JPJ,Li L,Denu JM,Puglielli L

PA3-16753 was used in Immunocytochemistry-immunofluorescence to reinforce the connection between aberrant cytosolic-to-endoplasmic reticulum acetyl-CoA flux and the development of an autistic-like phenotype.

Mon Apr 18 00:00:00 EDT 2022

Degeneration and regeneration of corneal nerves in response to HSV-1 infection.

Investigative ophthalmology & visual science

Chucair-Elliott AJ,Zheng M,Carr DJ

PA3-16753 was used in immunohistochemistry - frozen section to characterize corneal nerve network alterations induced by HSV-1 infection

Tue Jan 13 00:00:00 EST 2015

生物信息学

蛋白别名: 200 kDa neurofilament protein; heavy neurofilament protein; intermediate filament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC

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基因别名: CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH

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UniProt ID: (Human) P12036, (Mouse) P19246