Invitrogen

NFIA Polyclonal Antibody

NFIA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NFIA Antibody (PA5-79735) in IHC (P)

Immunohistochemistry analysis of NFIA on paraffin-embedded human mammary cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with NFIA polyclonal antibody (Product# PA5-79735) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). D... View More

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产品信息

PA5-79735

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

0.5-1 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence in the middle region of human NFIA (180-224aa AYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSG VFS).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2746850

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, alpha-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CCAAT-box-binding transcription factor; CTF; NF-I/A; NF1-A; NFI-A; Nuclear factor 1 A-type; nuclear factor 1/A; Nuclear factor I/A; TGGCA-binding protein

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基因别名: 1110047K16Rik; 9430022M17Rik; CTF; KIAA1439; NF-I/A; NF1-A; NF1A; Nf1l21; NFI-A; NFI-L; NFIA

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UniProt ID: (Human) Q12857, (Rat) P09414, (Mouse) Q02780

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Entrez Gene ID: (Human) 4774, (Rat) 25492, (Mouse) 18027

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding protein binding DNA-binding transcription factor gene-specific transcriptional regulator

参与通路

negative regulation of transcription from RNA polymerase II promoter DNA replication regulation of transcription, DNA-templated transcription from RNA polymerase II promoter viral genome replication positive regulation of transcription from RNA polymerase II promoter synapse maturation response to organic cyclic compound positive regulation of transcription, DNA-templated transcription, DNA-templated