Invitrogen

NFkB p100 Polyclonal Antibody

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

1个已发表图片

2 篇参考文献

查看其他22个NFkB p100抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 7

NFkB p100 Antibody (PA5-27340) in WB

Western Blot using NFkB p52 Polyclonal Antibody (Product # PA5-27340). Sample (30 µg of whole cell lysate). Lane A: Hela. Lane B: Hep G2 . 7.5% SDS PAGE. NFkB p100 antibody . NFkB p52 Polyclonal Antibody (Product # PA5-27340) diluted at 1:1,000.

NFkB p100 Antibody in Immunoprecipitation (IP)

NFkB p100 Antibody in Immunocytochemistry (ICC/IF)

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产品信息

PA5-27340

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:100-1:500

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 658 and 885 of Human NFkB p100/p52

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544816

产品详细信息

Recommended positive controls: A431, HeLa, HepG2, NIH-3T3 , YY-8103.

Predicted reactivity: Mouse (91%), Rat (90%), Bovine (85%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Novel Approach to Deliver Therapeutic Extracellular Vesicles Directly into the Mouse Kidney via Its Arterial Blood Supply.

Cells

Ullah M,Liu DD,Rai S,Razavi M,Choi J,Wang J,Concepcion W,Thakor AS

PA5-27340 was used in Quantitative reverse transcription PCR to develop in mice a reproducible microsurgical technique for the delivery of therapeutics directly into each kidney, via its arterial blood supply.

Fri Apr 10 00:00:00 EDT 2020

IKKα regulates human keratinocyte migration through surveillance of the redox environment.

Journal of cell science

Lisse TS,Rieger S

Published figure using NFkB p52 polyclonal antibody (Product # PA5-27340) in Immunofluorescence

Wed Mar 01 00:00:00 EST 2017

生物信息学

蛋白别名: DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Lyt10; NF kappaB2; NF-kB p100; NFKB, p52/p100 subunit; nuclear factor Kappa-B, subunit 2; Nuclear factor NF-kappa-B p100 subunit; nuclear factor NF-kappa-B p52 subunit; nuclear factor of Kappa light chain gene enhancer in B cells 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100; Oncogene Lyt-10; transcription factor NFKB2

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基因别名: CVID10; H2TF1; lyt; LYT-10; LYT10; NF-kappaB2; NF-kB2; NFKB2; p100; p49; p49/p100; p50B; p52

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UniProt ID: (Human) Q00653, (Mouse) Q9WTK5

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Entrez Gene ID: (Human) 4791, (Mouse) 18034

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding Rel homology transcription factor

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