Invitrogen

NFkB p65 Monoclonal Antibody (2A12A7)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

10个已发表图片

13 篇参考文献

查看其他90个NFkB p65抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

NFkB p65 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 20

NFkB p65 Antibody (33-9900) in IHC (P)

Immunohistochemistry analysis of NF-kappa-B (p65) showing staining in the Cytoplasm and Nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 m... View More

NFkB p65 Antibody in Immunocytochemistry (ICC/IF)

NFkB p65 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

33-9900

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5 - 1 µg/mL

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组织化学（悬浮细胞） (IHC (Free))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看3篇已发表文章 3篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

染色质免疫沉淀实验 (ChIP)

3 µg/mL

查看1篇已发表文章 1篇已发表文章

凝胶阻滞分析 (GS)

Assay-dependent

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

2A12A7

抗原

Recombinant protein (~175 amino acids) from the C-terminal portion of human NF-kappa-B (p65).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533153

靶标信息

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Efficacy of CU06-1004 via regulation of inflammation and endothelial permeability in LPS-induced acute lung injury.

Journal of inflammation (London, England)

Kim Y,Bae CR,Kim D,Kim H,Lee S,Zhang H,Noh M,Kim YM,Mochizuki N,Kwon YG

Published figure using NFkB p65 monoclonal antibody (Product # 33-9900) in Immunohistochemistry

Thu Apr 06 00:00:00 EDT 2023

Estrogen Receptor Beta Influences the Inflammatory p65 Cistrome in Colon Cancer Cells.

Frontiers in endocrinology

Indukuri R,Hases L,Archer A,Williams C

Published figure using NFkB p65 monoclonal antibody (Product # 33-9900) in ChIP assay

Fri Jan 07 00:00:00 EST 2022

NF-κB activation in retinal microglia is involved in the inflammatory and neovascularization signaling in laser-induced choroidal neovascularization in mice.

Experimental cell research

Hikage F,Lennikov A,Mukwaya A,Lachota M,Ida Y,Utheim TP,Chen DF,Huang H,Ohguro H

33-9900 was used in Immunohistochemistry to indicate that NF-κB activation in microglia and it's migration capacity is involved in the development of laser CNV in mice. Its suppression by NF-κB inhibition might be a promising therapeutic strategy for wet AMD.

Tue Jun 01 00:00:00 EDT 2021

Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression.

Molecular cell

Mejhert N,Kuruvilla L,Gabriel KR,Elliott SD,Guie MA,Wang H,Lai ZW,Lane EA,Christiano R,Danial NN,Farese RV,Walther TC

33-9900 was used in Immunocytochemistry-immunoflourescence to apply a genome-wide screening to identify genes that modulate lipid storage in macrophages, a cell type involved in metabolic diseases.

Thu Mar 19 00:00:00 EDT 2020

Epigenetic Suppression of HIV in Myeloid Cells by the BRD4-Selective Small Molecule Modulator ZL0580.

Journal of virology

Alamer E,Zhong C,Liu Z,Niu Q,Long F,Guo L,Gelman BB,Soong L,Zhou J,Hu H

33-9900 was used in Western Blotting to suggest a potential new approach that has utility in combination with ART to suppress residual HIV replication and/or HIV reactivation in CNS reservoirs, thereby reducing neuroinflammation and neurological disorders in HIV-infected individuals.

Mon May 18 00:00:00 EDT 2020

Cyclin F Downregulation Affects Epithelial-Mesenchymal Transition Increasing Proliferation and Migration of the A-375 Melanoma Cell Line.

Cancer management and research

Krajewski A,Gagat M,Mikołajczyk K,Izdebska M,Żuryń A,Grzanka A

33-9900 was used in Western Blotting to report that the downregulation of cyclin F in the A-375 melanoma cell line increases cell viability and colony formation in a cell cycle independent manner.

Tue Apr 19 00:00:00 EDT 2022

Structure-guided drug design identifies a BRD4-selective small molecule that suppresses HIV.

The Journal of clinical investigation

Niu Q,Liu Z,Alamer E,Fan X,Chen H,Endsley J,Gelman BB,Tian B,Kim JH,Michael NL,Robb ML,Ananworanich J,Zhou J,Hu H

Published figure using NFkB p65 monoclonal antibody (Product # 33-9900) in Western Blot

Mon Jul 22 00:00:00 EDT 2019

Role of p62/SQSTM1 beyond autophagy: a lesson learned from drug-induced toxicity in vitro.

British journal of pharmacology

Alegre F,Moragrega ÁB,Polo M,Marti-Rodrigo A,Esplugues JV,Blas-Garcia A,Apostolova N

Published figure using NFkB p65 monoclonal antibody (Product # 33-9900) in Western Blot

Thu Feb 01 00:00:00 EST 2018

A Single Nucleotide Polymorphism in the Vitamin D Receptor Gene Is Associated With Decreased Levels of the Protein and a Penetrating Pattern in Crohn's Disease.

Inflammatory bowel diseases

Gisbert-Ferrándiz L,Salvador P,Ortiz-Masiá D,Macías-Ceja DC,Orden S,Esplugues JV,Calatayud S,Hinojosa J,Barrachina MD,Hernández C

33-9900 was used in Western Blotting to analyze differences in vitamin D receptor expression levels among Crohn's Disease patients.

Fri Jun 08 00:00:00 EDT 2018

Glycocalyx Degradation Induces a Proinflammatory Phenotype and Increased Leukocyte Adhesion in Cultured Endothelial Cells under Flow.

PloS one

McDonald KK,Cooper S,Danielzak L,Leask RL

33-9900 was used in Immunocytochemistry-immunoflourescence to show that destabilisation of endothelial glycocalyx may be an early and defining feature in the initiation of atherosclerosis.

Fri Jun 30 00:00:00 EDT 2017

Antipsychotic drugs suppress the AKT/NF-κB pathway and regulate the differentiation of T-cell subsets.

Immunology letters

Chen ML,Tsai TC,Lin YY,Tsai YM,Wang LK,Lee MC,Tsai FM

Sun Oct 30 00:00:00 EDT 2011

Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies.

Journal of neuroinflammation

Herkenham M,Rathore P,Brown P,Listwak SJ

33-9900 was used in immunocytochemistry, immunohistochemistry (free floating), and western blot to examine specificity of the anti-NF-κB antibodies.

Fri Oct 14 00:00:00 EDT 2011

Sequence characteristics of functional siRNAs.

RNA (New York, N.Y.)

Jagla B,Aulner N,Kelly PD,Song D,Volchuk A,Zatorski A,Shum D,Mayer T,De Angelis DA,Ouerfelli O,Rutishauser U,Rothman JE

Published figure using NFkB p65 monoclonal antibody (Product # 33-9900) in Immunofluorescence

Wed Jun 01 00:00:00 EDT 2005

生物信息学

蛋白别名: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; REL A; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A

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基因别名: NFKB3; p65; RELA

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UniProt ID: (Human) Q04206, (Mouse) Q04207

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Entrez Gene ID: (Human) 5970, (Mouse) 19697

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功能

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding protein complex binding activating transcription factor binding phosphate ion binding identical protein binding protein homodimerization activity actinin binding histone deacetylase binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding NF-kappaB binding repressing transcription factor binding ankyrin repeat binding enzyme binding sequence-specific DNA binding Rel homology transcription factor

参与通路

NFkB p65 Monoclonal Antibody (2A12A7)

NFkB p65 Antibody (33-9900) in IHC (P)

产品信息

33-9900

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组织化学（悬浮细胞） (IHC (Free))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

染色质免疫沉淀实验 (ChIP)

凝胶阻滞分析 (GS)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

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NFkB p65 Monoclonal Antibody (2A12A7)

NFkB p65 Antibody (33-9900) in IHC (P)

产品信息

33-9900

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组织化学（悬浮细胞） (IHC (Free))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

染色质免疫沉淀 实验 (ChIP)

凝胶阻滞分析 (GS)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)