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Application
FIGURE: 1 / 5
NKCC1 Antibody (PA5-118800) in IHC (P)
Immunohistochemical analysis of NKCC1 in Mouse colorectal tissue using a NKCC1 polyclonal antibody (Product # PA5-118800). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Product Details
PA5-118800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SLC12A2(Accession P55011), corresponding to amino acid residues H226-T276.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2903300
Product Specific Information
Antibody detects endogenous levels of total SLC12A2.
Target Information
NKCC1 mediates sodium and chloride transport and reabsorption. NKCC1 is a membrane protein and is important in maintaining proper ionic balance and cell volume. NKCC1 is an electrically silent transporter system that mediates sodium and chloride reabsorption. Further, NKCC1 plays a vital role in the regulation of ionic balance and cell volume and is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for NKCC1. Diseases associated with SLC12A2 include Basosquamous Carcinoma and Metatypical Basal Cell Carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basolateral Na-K-Cl symporter; BSC2; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; hNKCC1; mNKCC1; Na-K-2Cl cotransporter 1; protein phosphatase 1, regulatory subunit 141; sodium/potassium/chloride cotransporters; sodium/potassium/chloride transporters; solute carrier family 12 (sodium/potassium/chloride transporter), member 2; solute carrier family 12 (sodium/potassium/chloride transporters), member 2; Solute carrier family 12 member 2; solute carrier family 12, member 2
Gene Aliases: 9330166H04Rik; BSC; BSC2; mBSC2; NKCC1; PPP1R141; SLC12A2; sy-ns
UniProt ID: (Human) P55011, (Mouse) P55012
Entrez Gene ID: (Human) 6558, (Rat) 83629, (Mouse) 20496
transport
ion transport
potassium ion transport
sodium ion transport
ammonium transport
transepithelial chloride transport
transepithelial ammonium transport
ammonium transmembrane transport
chloride transmembrane transport
chloride transport
hyperosmotic response
gamma-aminobutyric acid signaling pathway
aging
multicellular organism growth
positive regulation of cell volume
detection of mechanical stimulus involved in sensory perception of sound
branching involved in mammary gland duct morphogenesis
mammary duct terminal end bud growth
organic cation transport
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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