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NKX2-5 Monoclonal Antibody (3C1)

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抗体检测数据 (3)

NKX2-5 Antibody in Immunocytochemistry (ICC/IF)

NKX2-5 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

NKX2-5 Antibody (H00001482-M04) in ICC/IF

Immunofluorescence of monoclonal antibody to NKX2-5 on HeLa cell. Antibody concentration is 15 µg/mL.

NKX2-5 Antibody in Immunocytochemistry (ICC/IF)

NKX2-5 Antibody in Western Blot (WB)

NKX2-5 Antibody in Immunoprecipitation (IP)

Product Image Gallery

NKX2-5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of monoclonal antibody to NKX2-5 on HeLa cell. Antibody concentration is 15 µg/mL.

NKX2-5 Antibody in Western Blot (WB) — Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M04), clone 3C1.

Lane 1: NKX2-5 transfected lysate(34.9 KDa).
Lane 2: Non-transfected lysate.

NKX2-5 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of NKX2-5 transfected lysate using anti-NKX2-5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NKX2-5 MaxPab rabbit polyclonal antibody.

产品信息

H00001482-M04

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

-

免疫细胞化学 (ICC/IF)

15 µg/mL

-

酶联免疫吸附实验 (ELISA)

10 µg/mL

-

免疫沉淀 (IP)

1-5 µg/mL

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

3C1

抗原

NKX2-5 (NP_004378, 1 a.a. approximately 130 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

See Label

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Sequence of this protein is as follows: MFPSPALTPT PFSVKDILNL EQQQRSLAAA GELSARLEAT LAPSSCMLAA FKPEAYAGPE AAAPGLPELR AELGRAPSPA KCASAFPAAP AFYPRAYSDP DPAKDPRAEK KELCALQKAV ELEKTEADNA *

靶标信息

This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cardiac-specific homeobox; cardiac-specific homeobox 1; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein NKX 2-5; Homeobox protein Nkx-2.5; NK2 transcription factor related locus 5; NK2 transcription factor related, locus 5; NKX 2-5; Nkx-25; tinman paralog

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基因别名: CHNG5; CSX; CSX1; HLHS2; NKX2-5; NKX2.5; NKX2E; NKX4-1; VSD3

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UniProt ID: (Human) P52952

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Entrez Gene ID: (Human) 1482

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription cofactor activity transcriptional activator activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding serum response element binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity homeodomain transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter vasculogenesis heart looping heart morphogenesis outflow tract septum morphogenesis cardiac conduction system development bundle of His development Purkinje myocyte differentiation cardiac ventricle formation right ventricular cardiac muscle tissue morphogenesis ventricular trabecula myocardium morphogenesis apoptotic process involved in heart morphogenesis septum secundum development proepicardium development pulmonary myocardium development transcription from RNA polymerase II promoter adult heart development positive regulation of cell proliferation negative regulation of cardiac muscle cell apoptotic process positive regulation of transcription via serum response element binding positive regulation of sodium ion transport negative regulation of myotube differentiation hemopoiesis cell differentiation BMP signaling pathway thyroid gland development embryonic heart tube development negative regulation of apoptotic process sarcomere organization positive regulation of neuron differentiation positive regulation of heart contraction negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter spleen development positive regulation of cardioblast differentiation ventricular cardiac myofibril assembly cardiac muscle cell differentiation cardiac muscle tissue morphogenesis atrial cardiac muscle cell development ventricular cardiac muscle cell development regulation of cardiac muscle contraction pharyngeal system development cardiac muscle cell proliferation regulation of cardiac muscle cell proliferation cardiac muscle contraction canonical Wnt signaling pathway positive regulation of transcription initiation from RNA polymerase II promoter heart trabecula formation ventricular septum morphogenesis atrial septum morphogenesis atrioventricular node cell development atrioventricular node cell fate commitment embryonic heart tube left/right pattern formation negative regulation of canonical Wnt signaling pathway regulation of cardiac conduction