Invitrogen

NME2 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他26个NME2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (4)
高级验证 (1)

图: 1 / 5

NME2 Antibody (PA5-27488) in WB

Western Blot was performed using Anti-NME2 Polyclonal Antibody (Product # PA5-27488) and a 17 and 30 kDa band corresponding to Nucleoside diphosphate kinase B was observed across cell lines and tissues tested . Nuclear enriched extracts (30 µg lysate) of A549 (Lane 1), HeLa (Lane 2), SH-SY5Y (Lane 3), PC-3 (Lane 4), Mouse Liver (Lane 5) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (... View More

Product Image Gallery

NME2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with Nucleoside diphosphate kinase B siRNA and decrease in signal intensity was observed in Western Blot application using Anti-NME2 Polyclonal Antibody (Product # PA5-27488). {KD}

产品信息

PA5-27488

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 105 of Human nm23-H2

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544964

产品详细信息

Recommended positive controls: A549, H1299, HCT116, mouse brain.^M
^M
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (89%), Dog (93%), Pig (95%), Rabbit (100%), Chicken (94%), Rhesus Monkey (98%), Bovine (97%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Nucleoside diphosphate kinase B is an enzyme that in humans is encoded by the NME2 gene. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein consisting of sequence sharing identity with each individual gene product.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: 2.7.13.3; C-myc purine-binding transcription factor PUF; c-myc transcription factor; epididymis secretory sperm binding protein Li 155an; expressed in non-metastatic cells 2 protein; HEL-S-155an; Histidine protein kinase NDKB; NDK B; NDP kinase B; nm23-H2; nm23-M2; non-metastatic cells 2, protein (NM23) expressed in; non-metastatic cells 2, protein (NM23B) expressed in; Nucleoside diphosphate kinase B; nucleoside-diphosphate kinase 2; nucleotide diphosphate kinase B; P18

查看更多收起

基因别名: NDKB; NDPK-B; NDPKB; NM23-H2; nm23-M2; NM23B; NME2; PUF

查看更多收起

UniProt ID: (Human) P22392, (Mouse) Q01768

查看更多收起

Entrez Gene ID: (Human) 4831, (Mouse) 18103

查看更多收起

功能