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NR4A1 Antibody (PA5-87511) in WB
Western blot analysis of NR4A1 in extracts of SH-SY5Y cells. Samples were incubated with NR4A1 Polyclonal antibody (Product # PA5-87511) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-87511
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NUR77 (NP_7751801)
偶联物
形式
Liquid
浓度
0.62 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804212
产品详细信息
Immunogen sequence: PSLAQSPLKL FPSQATHQLG EGESYSMPTA FPGLAPTSPH LEGSGILDTP VTSTKARSGA PGGSEGRCAV CGDNASCQHY GVRTCEGCKG FFKRTVQKNA K
Positive Samples: SH-SY5Y; Cellular Location: Cytoplasm, Nucleus
靶标信息
NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Early response protein NAK1; growth factor-inducible nuclear protein N10; hormone receptor; MGC9485; nerve growth factor IB nuclear receptor variant 1; Nuclear hormone receptor NUR/77; nuclear receptor subfamily 4, group A, member 1; Nuclear receptor subfamily 4immunitygroup A member 1; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; Orphan receptor tr3; ST-59; steroid receptor TR3; Testicular receptor 3; TR3 orphan receptor
基因别名: GFRP1; HMR; N10; NAK-1; NAK1; NGFIB; NP10; NR4A1; NUR77; TR3
UniProt ID: (Human) P22736
Entrez Gene ID: (Human) 3164
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
protein heterodimerization activity
C4 zinc finger nuclear receptor
positive regulation of endothelial cell proliferation
cell migration involved in sprouting angiogenesis
transcription initiation from RNA polymerase II promoter
signal transduction
intracellular receptor signaling pathway
endothelial cell chemotaxis
cellular response to vascular endothelial growth factor stimulus
steroid hormone mediated signaling pathway
cellular response to fibroblast growth factor stimulus
fat cell differentiation
negative regulation of cell cycle
positive regulation of transcription from RNA polymerase II promoter
regulation of type B pancreatic cell proliferation
cellular response to corticotropin-releasing hormone stimulus
