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NSUN4 Antibody (MA5-42533) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of NSUN4 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with NSUN4 Monoclonal Antibody (Product # MA5-42533) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
产品信息
MA5-42533
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4G3
抗原
Recombinant protein within human NSUN4 aa 1-384
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Protein G
保存液
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2911674
产品详细信息
Positive Control: MCF-7 cell lysates, MCF-7.^M
^M
Subcellular Location: Mitochondrion.
靶标信息
NSUN4 is a 5-methylcytosine rRNA methytransferase that is involved in mitochondiral ribosome assembly. More specifically NSUN4 is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA. This function is independent of MTREFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). NSUN4 is targeted to LSU by MTREFD2/MTERF4 and is involved in the final step in ribosome biogenesis to ensure that SSU and LSU are assembled.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5-methylcytosine rRNA methyltransferase NSUN4; 5-methylcytosine tRNA methyltransferase NSUN4; NOL1/NOP2/Sun domain family member 4; NOP2/Sun domain family, member 4; putative methyltransferase NSUN4; RP4-603I14.2; sperm head and tail associated protein
基因别名: NSUN4; SHTAP
UniProt ID: (Human) Q96CB9
Entrez Gene ID: (Human) 387338
