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产品信息
PA5-100651
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NUDT16(Accession Q96DE0), corresponding to amino acid residues L73-V123.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2850156
产品详细信息
Antibody detects endogenous levels of total NUDT16.
靶标信息
RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms (PubMed:20385596, PubMed:26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP (PubMed:20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions (PubMed:20385596). [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: IDP phosphatase; IDPase; Inosine diphosphate phosphatase; m7GpppN-mRNA hydrolase; Nucleoside diphosphate-linked moiety X motif 16; nudix (nucleoside diphosphate linked moiety X)-type motif 16; Nudix hydrolase 16; Nudix motif 16; testicular tissue protein Li 129; U8 snoRNA-binding protein H29K; U8 snoRNA-decapping enzyme
基因别名: NUDT16
UniProt ID: (Human) Q96DE0
Entrez Gene ID: (Human) 131870
magnesium ion binding
mRNA binding
GTP binding
metalloexopeptidase activity
manganese ion binding
snoRNA binding
dITP diphosphatase activity
identical protein binding
protein homodimerization activity
m7G(5')pppN diphosphatase activity
cobalt ion binding
inosine-diphosphatase activity
dIDP diphosphatase activity
nucleotide phosphatase activity, acting on free nucleotides
XTP binding
ITP binding
adenosine to inosine editing
mRNA catabolic process
proteolysis
positive regulation of cell proliferation
snoRNA catabolic process
dephosphorylation
nucleobase-containing small molecule catabolic process
dITP catabolic process
IDP catabolic process
positive regulation of cell cycle process
XDP catabolic process
negative regulation of rRNA processing
positive regulation of double-strand break repair
