Invitrogen

NUP107 Polyclonal Antibody

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NUP107 Antibody (PA5-30774) in WB

Western blot was performed using Anti-NUP107 Polyclonal Antibody (Product # PA5-30774) and a 107 kDa band corresponding to NUP107 was observed across all the cell lines tested along with an uncharacterized band (*) at ~ 50 kDa. Modified whole cell extracts (1% SDS) (30 µg lysate) of HEK-293 (Lane 1), MCF7 (Lane 2) and PC-3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Pr... View More

NUP107 Antibody in Immunoprecipitation (IP)

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产品信息

PA5-30774

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:3,000

免疫细胞化学 (ICC/IF)

- -

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 205 and 449 of Human NUP107

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.44 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2548248

产品详细信息

Recommended positive controls: A549, HeLa, HepG2, HCT116.

Predicted reactivity: Mouse (95%), Rat (95%), Zebrafish (81%), Xenopus laevis (85%), Chicken (88%), Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-107 is part of the hetero-oligomeric complex that also contains NUP160, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP107 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP107 may be an important drug target in HIV treatment. Multiple isoforms of NUP107 are known to exist.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。